PXD030893

PXD030893 is an original dataset announced via ProteomeXchange.

Title	Analysis of oncogenic KRAS impact on the phospho-proteome of PDAC cells.
Description	To reveal the impact of mutant KRAS on the phospho-proteome of Pancreatic Ductal Adenocarcinoma (PDAC) cells, we carried out a quantitative phospho-proteomic analysis of tumour cells isolated from an inducible mouse model of PDAC (iKras PDAC) (Ying et al., 2012). In this model, oncogenic Kras (G12D) expression can be controlled by administration of doxycycline (Dox). Tandem Mass Tagging (TMT) was employed for quantitative analysis of Dox induced (24hrs) vs. non-induced iKras-PDAC cells. To assess the impact of ERK1/2 signalling, we also analysed cells induced with Dox in presence of Trametinib (10nM), a potent and specific inhibitor of ERK1/2 signalling. A total of 3 biological replicates per condition were assessed, with all samples barcoded and pooled together using TMT 10plex labelling kit (Thermo). To enrich for phospho-peptides, TiO phospho-peptide enrichment was performed. Both the phospho-enriched as well as the total peptide pools were analysed by mass spectrometry and the phopho to total ratio was calculated as a specific measure of phosphorylation change.
HostingRepository	PRIDE
AnnounceDate	2023-02-12
AnnouncementXML	Submission_2023-02-12_07:37:23.002.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FarazMardakheh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-11 01:55:54	ID requested
⏵ 1	2023-02-12 07:37:23	announced

Dataset with its publication pending

submitter keyword: KRAS

TMT

Phospho-proteomics

PDAC

ERK

Faraz K.Mardakheh
contact affiliation	Centre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London Charterhouse Square London EC1M 6BQ U.K.
contact email	f.mardakheh@qmul.ac.uk
lab head
FarazMardakheh
contact affiliation	Barts Cancer Institute
contact email	f.mardakheh@qmul.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030893
     1. Label: PRIDE project
     2. Name: Analysis of oncogenic KRAS impact on the phospho-proteome of PDAC cells.