PXD030871

PXD030871 is an original dataset announced via ProteomeXchange.

Title	Quantitative XL-MS Analysis of HHARI in the presence or absence of 4EHP
Description	RING-Between-RING (RBR) E3 ligases mediate ubiquitin transfer through an obligate E3- ubiquitin thioester intermediately prior to substrate ubiquitination. While RBRs share a conserved catalytic module, substrate recruitment mechanisms remain enigmatic and the relevant domains have yet to be identified for any member of the class. Here we characterize the interaction between the auto-inhibited RBR, HHARI (AriH1), and its target protein, 4EHP, using quantitative XL-MS.
HostingRepository	PanoramaPublic
AnnounceDate	2024-07-26
AnnouncementXML	Submission_2024-07-26_15:39:51.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Alex Zelter
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-10 18:53:56	ID requested
⏵ 1	2024-07-26 15:39:52	announced

Reiter KH, Zelter A, Janowska MK, Riffle M, Shulman N, MacLean BX, Tamura K, Chambers MC, MacCoss MJ, Davis TN, Guttman M, Brzovic PS, Klevit RE, Cullin-independent recognition of HHARI substrates by a dynamic RBR catalytic domain. Structure, 30(9):1269-1284.e6(2022) [pubmed]

submitter keyword: Cross-linking Mass Spectrometry Kojak ProXL Skyline Cullin HHARI RBR E3 ligase ubiquitin

Rachel E. Klevit
contact affiliation	University of Washington
contact email	klevit@uw.edu
lab head
Alex Zelter
contact affiliation	University of Washington
contact email	azelter@uw.edu
dataset submitter

Panorama Public dataset URI