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PXD030864

PXD030864 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGenetically targeted proteomics identify compositional and phosphoproteome trajectories of corticostriatal projections across postnatal development
DescriptionAxonal development in mammals begins in the embryonic stage and continues postnatally. During early postnatal period, proteomic landscape of axons changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Studying axonal proteome across development in complex tissue remains challenging. Axonal compartments from the brain cannot be isolated without substantial losses. Axons from different circuits are intermingled; isolation techniques that do not resolve cell types likely mask neuronal class specific features. Here, we create a Cre-dependent APEX2 reporter mouse line and demonstrate its utility to map cell-type specific proteome of corticostriatal projections across postnatal development. We define temporal patterns of axonal proteome and phosphoproteome levels, and their relevance to neurodegenerative and psychiatric diseases. This analysis workflow also identifies proline-directed kinases and phosphosites as major regulators for corticostriatal development. Altogether, APEX2 transgenic reporter-based proximity labeling approach offers a flexible strategy for mapping subcellular proteome, with cell type specificity, across neurodevelopment.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:01:33.214.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVasin Dumrongprechachan
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListbiotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-10 01:05:36ID requested
12022-10-26 08:56:35announced
22023-11-14 08:01:34announced2023-11-14: Updated project metadata.
Publication List
Dumrongprechachan V, Salisbury RB, Butler L, MacDonald ML, Kozorovitskiy Y, Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment. Elife, 11():(2022) [pubmed]
Keyword List
submitter keyword: axon development, proximity labeling,APEX2
Contact List
Yevgenia Kozorovitskiy
contact affiliationDepartment of Neurobiology, Northwestern University, Evanston, IL, USA
contact emailyevgenia.kozorovitskiy@northwestern.edu
lab head
Vasin Dumrongprechachan
contact affiliationNorthwestern University
contact emailvasindumrongprechachan2015@u.northwestern.edu
dataset submitter
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Dataset FTP location
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