PXD030864 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Genetically targeted proteomics identify compositional and phosphoproteome trajectories of corticostriatal projections across postnatal development |
Description | Axonal development in mammals begins in the embryonic stage and continues postnatally. During early postnatal period, proteomic landscape of axons changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Studying axonal proteome across development in complex tissue remains challenging. Axonal compartments from the brain cannot be isolated without substantial losses. Axons from different circuits are intermingled; isolation techniques that do not resolve cell types likely mask neuronal class specific features. Here, we create a Cre-dependent APEX2 reporter mouse line and demonstrate its utility to map cell-type specific proteome of corticostriatal projections across postnatal development. We define temporal patterns of axonal proteome and phosphoproteome levels, and their relevance to neurodegenerative and psychiatric diseases. This analysis workflow also identifies proline-directed kinases and phosphosites as major regulators for corticostriatal development. Altogether, APEX2 transgenic reporter-based proximity labeling approach offers a flexible strategy for mapping subcellular proteome, with cell type specificity, across neurodevelopment. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:01:33.214.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vasin Dumrongprechachan |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | biotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-10 01:05:36 | ID requested | |
1 | 2022-10-26 08:56:35 | announced | |
⏵ 2 | 2023-11-14 08:01:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dumrongprechachan V, Salisbury RB, Butler L, MacDonald ML, Kozorovitskiy Y, Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment. Elife, 11():(2022) [pubmed] |
Keyword List
submitter keyword: axon development, proximity labeling,APEX2 |
Contact List
Yevgenia Kozorovitskiy |
contact affiliation | Department of Neurobiology, Northwestern University, Evanston, IL, USA |
contact email | yevgenia.kozorovitskiy@northwestern.edu |
lab head | |
Vasin Dumrongprechachan |
contact affiliation | Northwestern University |
contact email | vasindumrongprechachan2015@u.northwestern.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030864
- Label: PRIDE project
- Name: Genetically targeted proteomics identify compositional and phosphoproteome trajectories of corticostriatal projections across postnatal development