PXD030856

PXD030856 is an original dataset announced via ProteomeXchange.

Title	Identification of New ATG8s-binding Proteins with Canonical LC3-interacting Region in Autophagosomes of Barley Callus
Description	Autophagy is essential to maintain cellular homeostasis for normal cell growth and development. In selective autophagy, ATG8 plays a crucial role in cargo target recognition by binding to various adaptors and receptors with the ATG8-interacting motif, also known as the LC3-interacting region (LIR). However, the process of autophagy in the callus, as a proliferating cell type, is largely unknown. In this study, we overexpressed green fluorescent protein (GFP)-ATG8a and GFP-ATG8b transgenic barley callus and checked their autophagic activities. We identified five new ATG8 candidate interactors containing the canonical LIR motif by using immunoprecipitation coupled with mass spectrometry: RPP3, COPE, NCLN, RAE1, and CTSL. The binding activities between these candidate interactors and ATG8 were further demonstrated in the punctate structure. Notably, RPP3 was colocalized in ATG8-labeled autophagosomes under tunicamycin-induced ER stress. GST pull-down assays showed that the interaction between RPP3 and ATG8 could be prevented by mutating the LIRs region of RPP3 or the LIR docking site (LDS) of ATG8, suggesting that RPP3 directly interacted with ATG8 in an LIR-dependent manner via the LDS. Our findings would provide the basis for further investigations on novel receptors and functions of autophagy in plants, especially in the physiological state of cell de-differentiation.
HostingRepository	iProX
AnnounceDate	2022-01-10
AnnouncementXML	Submission_2023-12-24_20:29:31.541.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bu Erte
SpeciesList	scientific name: Hordeum vulgare subsp. vulgare; NCBI TaxID: 112509;
ModificationList	No PTMs are included in the dataset
Instrument	mass spectrometry/mass spectrometry

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-09 23:49:59	ID requested
⏵ 1	2023-12-24 20:29:32	announced

B B, Zeng Z, Zhou C, Lian G, Guo F, Wang J, Han N, Zhu M, Bian H, Identification of New ATG8s-Binding Proteins with Canonical LC3-Interacting Region in Autophagosomes of Barley Callus. Plant Cell Physiol, ():(2022) [pubmed]

submitter keyword: Autophagy, ATG8, barley, callus, interactor, LIR

Hongwu Bian
contact affiliation	Zhejiang University
contact email	hwbian@zju.edu.cn
lab head
Bu Erte
contact affiliation	Zhejiang University
contact email	buertecn@163.com
dataset submitter

iProX dataset URI