PXD030856
PXD030856 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of New ATG8s-binding Proteins with Canonical LC3-interacting Region in Autophagosomes of Barley Callus |
Description | Autophagy is essential to maintain cellular homeostasis for normal cell growth and development. In selective autophagy, ATG8 plays a crucial role in cargo target recognition by binding to various adaptors and receptors with the ATG8-interacting motif, also known as the LC3-interacting region (LIR). However, the process of autophagy in the callus, as a proliferating cell type, is largely unknown. In this study, we overexpressed green fluorescent protein (GFP)-ATG8a and GFP-ATG8b transgenic barley callus and checked their autophagic activities. We identified five new ATG8 candidate interactors containing the canonical LIR motif by using immunoprecipitation coupled with mass spectrometry: RPP3, COPE, NCLN, RAE1, and CTSL. The binding activities between these candidate interactors and ATG8 were further demonstrated in the punctate structure. Notably, RPP3 was colocalized in ATG8-labeled autophagosomes under tunicamycin-induced ER stress. GST pull-down assays showed that the interaction between RPP3 and ATG8 could be prevented by mutating the LIRs region of RPP3 or the LIR docking site (LDS) of ATG8, suggesting that RPP3 directly interacted with ATG8 in an LIR-dependent manner via the LDS. Our findings would provide the basis for further investigations on novel receptors and functions of autophagy in plants, especially in the physiological state of cell de-differentiation. |
HostingRepository | iProX |
AnnounceDate | 2022-01-10 |
AnnouncementXML | Submission_2023-12-24_20:29:31.541.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bu Erte |
SpeciesList | scientific name: Hordeum vulgare subsp. vulgare; NCBI TaxID: 112509; |
ModificationList | No PTMs are included in the dataset |
Instrument | mass spectrometry/mass spectrometry |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-01-09 23:49:59 | ID requested | |
⏵ 1 | 2023-12-24 20:29:32 | announced |
Publication List
B B, Zeng Z, Zhou C, Lian G, Guo F, Wang J, Han N, Zhu M, Bian H, Identification of New ATG8s-Binding Proteins with Canonical LC3-Interacting Region in Autophagosomes of Barley Callus. Plant Cell Physiol, ():(2022) [pubmed] |
Keyword List
submitter keyword: Autophagy, ATG8, barley, callus, interactor, LIR |
Contact List
Hongwu Bian | |
---|---|
contact affiliation | Zhejiang University |
contact email | hwbian@zju.edu.cn |
lab head | |
Bu Erte | |
contact affiliation | Zhejiang University |
contact email | buertecn@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |