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PXD030856

PXD030856 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of New ATG8s-binding Proteins with Canonical LC3-interacting Region in Autophagosomes of Barley Callus
DescriptionAutophagy is essential to maintain cellular homeostasis for normal cell growth and development. In selective autophagy, ATG8 plays a crucial role in cargo target recognition by binding to various adaptors and receptors with the ATG8-interacting motif, also known as the LC3-interacting region (LIR). However, the process of autophagy in the callus, as a proliferating cell type, is largely unknown. In this study, we overexpressed green fluorescent protein (GFP)-ATG8a and GFP-ATG8b transgenic barley callus and checked their autophagic activities. We identified five new ATG8 candidate interactors containing the canonical LIR motif by using immunoprecipitation coupled with mass spectrometry: RPP3, COPE, NCLN, RAE1, and CTSL. The binding activities between these candidate interactors and ATG8 were further demonstrated in the punctate structure. Notably, RPP3 was colocalized in ATG8-labeled autophagosomes under tunicamycin-induced ER stress. GST pull-down assays showed that the interaction between RPP3 and ATG8 could be prevented by mutating the LIRs region of RPP3 or the LIR docking site (LDS) of ATG8, suggesting that RPP3 directly interacted with ATG8 in an LIR-dependent manner via the LDS. Our findings would provide the basis for further investigations on novel receptors and functions of autophagy in plants, especially in the physiological state of cell de-differentiation.
HostingRepositoryiProX
AnnounceDate2022-01-10
AnnouncementXMLSubmission_2023-12-24_20:29:31.541.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBu Erte
SpeciesList scientific name: Hordeum vulgare subsp. vulgare; NCBI TaxID: 112509;
ModificationListNo PTMs are included in the dataset
Instrumentmass spectrometry/mass spectrometry
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-09 23:49:59ID requested
12023-12-24 20:29:32announced
Publication List
B B, Zeng Z, Zhou C, Lian G, Guo F, Wang J, Han N, Zhu M, Bian H, Identification of New ATG8s-Binding Proteins with Canonical LC3-Interacting Region in Autophagosomes of Barley Callus. Plant Cell Physiol, ():(2022) [pubmed]
Keyword List
submitter keyword: Autophagy, ATG8, barley, callus, interactor, LIR
Contact List
Hongwu Bian
contact affiliationZhejiang University
contact emailhwbian@zju.edu.cn
lab head
Bu Erte
contact affiliationZhejiang University
contact emailbuertecn@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI