PXD030847

PXD030847 is an original dataset announced via ProteomeXchange.

Title	Identification and characterization of β-ODAP synthase in grass pea
Description	Grass pea seeds and seedlings protein extracts were chromatographically fractionated. To identify the β-ODAP synthase enzyme, active fractions, as determined by a colorimetric assay that detects the presence of a derivative of free L-α,β-diaminopropionic acid (L-DAPA), were subjected to tryptic digestion and LC-MS/MS and searched against a database containing translated sequences from a long-read PacBio mRNA sequencing of grass pea seeds and seedlings.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_06:48:23.466.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alon Savidor
SpeciesList	scientific name: Lathyrus sativus; NCBI TaxID: 3860;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-09 11:43:36	ID requested
⏵ 1	2022-05-19 06:48:24	announced

Goldsmith M, Barad S, Knafo M, Savidor A, Ben-Dor S, Brandis A, Mehlman T, Peleg Y, Albeck S, Dym O, Ben-Zeev E, Barbole RS, Aharoni A, Reich Z, -ODAP in grass pea. J Biol Chem, 298(5):101806(2022) [pubmed]

submitter keyword: Lathyrus sativus, grass pea, toxin, beta-ODAP synthetase, trascriptome, proteomics

Ziv Reich
contact affiliation	Department of Biomolecular Sciences Faculty of Biochemistry Weizmann Institute of Science Rehovot, Israel
contact email	ziv.reich@weizmann.ac.il
lab head
Alon Savidor
contact affiliation	The Weizmann Institute of Science
contact email	alon.savidor@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030847

PRIDE project URI

• PRIDE
  1. PXD030847
     1. Label: PRIDE project
     2. Name: Identification and characterization of β-ODAP synthase in grass pea