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PXD030845

PXD030845 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePytheas SARS-CoV-2 spike protein mRNA sample analysis
DescriptionMass spectrometry remains an important method for analysis of modified nucleosides ubiquitously present in cellular RNAs, in particular for ribosomal and transfer RNAs that play crucial roles in mRNA translation and decoding. Furthermore, modifications have effect on the lifetimes of nucleic acids in plasma and cells and are consequently incorporated into RNA therapeutics. To provide an analytical tool for sequence characterization of modified RNAs, we developed Pytheas, an open-source software package for automated analysis of tandem MS data for RNA. This dataset contains the analysis of 14N and 15N-labeled synthetic mRNA of the SARS-CoV-2 spike protein. All uridines were modified to m1Ψ.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:38:34.686.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuigi D'Ascenzo
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
Instrument6520A Quadrupole Time-of-Flight LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-09 11:40:46ID requested
12024-10-08 11:38:35announced
Publication List
10.1038/S41467-022-30057-5;
10.1038/s41467-022-30057-5;
D'Ascenzo L, Popova AM, Abernathy S, Sheng K, Limbach PA, Williamson JR, Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry. Nat Commun, 13(1):2424(2022) [pubmed]
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19
submitter keyword: isotope labeling, SARS-CoV-2 spike protein,Pytheas, mRNA
Contact List
James R. Williamson
contact affiliationDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, United States
contact emailjrwill@scripps.edu
lab head
Luigi D'Ascenzo
contact affiliationThe Scripps Research Institute
contact emaildascenzoluigi@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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