PXD030844
PXD030844 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pytheas S. cerevisiae tRNA sample analysis |
Description | Mass spectrometry remains an important method for analysis of modified nucleosides ubiquitously present in cellular RNAs, in particular for ribosomal and transfer RNAs that play crucial roles in mRNA translation and decoding. Furthermore, modifications have effect on the lifetimes of nucleic acids in plasma and cells and are consequently incorporated into RNA therapeutics. To provide an analytical tool for sequence characterization of modified RNAs, we developed Pytheas, an open-source software package for automated analysis of tandem MS data for RNA. This dataset contains the analysis of a mixture of S. cerevisiae tRNAs, decorated with a variety of complex RNA modifications. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:38:34.593.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luigi D'Ascenzo |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | 6520A Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-01-09 11:39:50 | ID requested | |
⏵ 1 | 2024-10-08 11:38:36 | announced |
Publication List
10.1038/S41467-022-30057-5; |
10.1038/s41467-022-30057-5; |
D'Ascenzo L, Popova AM, Abernathy S, Sheng K, Limbach PA, Williamson JR, Pytheas: a software package for the automated analysis of RNA sequences and modifications via tandem mass spectrometry. Nat Commun, 13(1):2424(2022) [pubmed] |
Keyword List
submitter keyword: RNA modifications, yeast,Pytheas |
Contact List
Jamer R. Williamson | |
---|---|
contact affiliation | Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, United States |
contact email | jrwill@scripps.edu |
lab head | |
Luigi D'Ascenzo | |
contact affiliation | The Scripps Research Institute |
contact email | dascenzoluigi@gmail.com |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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