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PXD030843

PXD030843 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEffects of plasticizers on adult human adipocytes (SGBS) using global proteomics
DescriptionSGBS cells were used as a model system to investigate the cellular mode of action of a variety of plasticizers in human adipocytes. In brief, cells were cultured at 5% CO2 and 37°C in 95% humidity. Cells of generation 40 and passage 3 after thawing were grown to confluence with DMEM/F12 containing 33 µM biotin, 17 µM pantothenate, 100 U/l penicillin, and 0.1 mg/l streptomycin (basal medium) supplemented with 10% FCS (Gibco, Carlsbad, CA, USA). According to the standard differentiation protocol, differentiation was initiated (day 0) after changing to serum-free basal medium supplemented with 0.1 μM cortisol, 0.01 mg/ml apo-transferrin, 0.2 nM triiodothyronine, and 20 nM human insulin (differentiation medium) with the addition of 2 µM rosiglitazone, 25 nM dexamethasone, and 200 µM 3-isobutyl-1-methylxanthine for the first four days. SGBS cells were first differentiated according to the standard protocol for 12 days. Obtained adult adipocytes were then exposed to the plasticizers for 8 days with medium changes every second day. The control contained equivalent amounts of solvent (0.01% MeOH). Additinally, a control on day 12 before exposure was sampled. All SGBS cell culture experiments were conducted in quadruplets.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:54:28.494.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030843
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexandra Schaffert
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-09 11:38:44ID requested
12022-06-19 18:06:57announced
22023-11-14 08:54:28announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD030843;
Keyword List
submitter keyword: xenobiotics, TMT, adipocytes,global proteomics, untargeted proteomics, SGBS
Contact List
Kristin Schubert
contact affiliationDepartment of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
contact emailkristin.schubert@ufz.de
lab head
Alexandra Schaffert
contact affiliationHelmholtz-Centre for Environmental Research
contact emailalexandra.schaffert@ufz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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