PXD030843

PXD030843 is an original dataset announced via ProteomeXchange.

Title	Effects of plasticizers on adult human adipocytes (SGBS) using global proteomics
Description	SGBS cells were used as a model system to investigate the cellular mode of action of a variety of plasticizers in human adipocytes. In brief, cells were cultured at 5% CO2 and 37°C in 95% humidity. Cells of generation 40 and passage 3 after thawing were grown to confluence with DMEM/F12 containing 33 µM biotin, 17 µM pantothenate, 100 U/l penicillin, and 0.1 mg/l streptomycin (basal medium) supplemented with 10% FCS (Gibco, Carlsbad, CA, USA). According to the standard differentiation protocol, differentiation was initiated (day 0) after changing to serum-free basal medium supplemented with 0.1 μM cortisol, 0.01 mg/ml apo-transferrin, 0.2 nM triiodothyronine, and 20 nM human insulin (differentiation medium) with the addition of 2 µM rosiglitazone, 25 nM dexamethasone, and 200 µM 3-isobutyl-1-methylxanthine for the first four days. SGBS cells were first differentiated according to the standard protocol for 12 days. Obtained adult adipocytes were then exposed to the plasticizers for 8 days with medium changes every second day. The control contained equivalent amounts of solvent (0.01% MeOH). Additinally, a control on day 12 before exposure was sampled. All SGBS cell culture experiments were conducted in quadruplets.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:28.494.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030843
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexandra Schaffert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-09 11:38:44	ID requested
1	2022-06-19 18:06:57	announced
⏵ 2	2023-11-14 08:54:28	announced	2023-11-14: Updated project metadata.

10.6019/PXD030843;

submitter keyword: xenobiotics, TMT, adipocytes,global proteomics, untargeted proteomics, SGBS

Kristin Schubert
contact affiliation	Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany
contact email	kristin.schubert@ufz.de
lab head
Alexandra Schaffert
contact affiliation	Helmholtz-Centre for Environmental Research
contact email	alexandra.schaffert@ufz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030843
     1. Label: PRIDE project
     2. Name: Effects of plasticizers on adult human adipocytes (SGBS) using global proteomics