PXD030843 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Effects of plasticizers on adult human adipocytes (SGBS) using global proteomics |
Description | SGBS cells were used as a model system to investigate the cellular mode of action of a variety of plasticizers in human adipocytes. In brief, cells were cultured at 5% CO2 and 37°C in 95% humidity. Cells of generation 40 and passage 3 after thawing were grown to confluence with DMEM/F12 containing 33 µM biotin, 17 µM pantothenate, 100 U/l penicillin, and 0.1 mg/l streptomycin (basal medium) supplemented with 10% FCS (Gibco, Carlsbad, CA, USA). According to the standard differentiation protocol, differentiation was initiated (day 0) after changing to serum-free basal medium supplemented with 0.1 μM cortisol, 0.01 mg/ml apo-transferrin, 0.2 nM triiodothyronine, and 20 nM human insulin (differentiation medium) with the addition of 2 µM rosiglitazone, 25 nM dexamethasone, and 200 µM 3-isobutyl-1-methylxanthine for the first four days. SGBS cells were first differentiated according to the standard protocol for 12 days. Obtained adult adipocytes were then exposed to the plasticizers for 8 days with medium changes every second day. The control contained equivalent amounts of solvent (0.01% MeOH). Additinally, a control on day 12 before exposure was sampled. All SGBS cell culture experiments were conducted in quadruplets. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:54:28.494.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD030843 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexandra Schaffert |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-09 11:38:44 | ID requested | |
1 | 2022-06-19 18:06:57 | announced | |
⏵ 2 | 2023-11-14 08:54:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: xenobiotics, TMT, adipocytes,global proteomics, untargeted proteomics, SGBS |
Contact List
Kristin Schubert |
contact affiliation | Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany |
contact email | kristin.schubert@ufz.de |
lab head | |
Alexandra Schaffert |
contact affiliation | Helmholtz-Centre for Environmental Research |
contact email | alexandra.schaffert@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030843
- Label: PRIDE project
- Name: Effects of plasticizers on adult human adipocytes (SGBS) using global proteomics