PXD030803

PXD030803 is an original dataset announced via ProteomeXchange.

Title	Paladin, a pseudo-phosphatase overexpressed in colon cancer, is required for actin polymerization and liver metastasis dissemination
Description	Colorectal cancer remains a public health issue and most of colon cancer patients succumb to the development of metastases. Using a specific protocol, we identified paladin as a potential colon cancer liver metastases biomarker. We then explored the biological function of paladin in colon cancer cells. We discovered that paladin is required for cell migration and metastasis. We reported that paladin depletion altered the phospho-proteome within colon cancer cells. Thanks to immuno-precipitation experiments, we demonstrated that paladin was interacting with SSH-1, a phosphatase involved in colon cancer metastasis. Finally, we showed that paladin depletion in cancer cells altered the interaction between SSH-1 and actin, which results in a less dynamic actin cytoskeleton. In conclusion, paladin is an under undervalued protein in oncology participating in actin cytoskeleton remodelling and necessary for an efficient cancer cell migration
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:05.365.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030803
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-07 02:42:36	ID requested
1	2022-07-27 02:50:10	announced
⏵ 2	2023-11-14 08:55:05	announced	2023-11-14: Updated project metadata.

10.6019/PXD030803;

submitter keyword: phosphoproteome, actin cytoskeleton,colon cancer, paladin

Olivier Peulen
contact affiliation	Metastasis Research Laboratory Liège université GIGA institute ULIEGE-CHU-B23 +4, 4000 Liège Belgium
contact email	olivier.peulen@uliege.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD030803

PRIDE project URI

• PRIDE
  1. PXD030803
     1. Label: PRIDE project
     2. Name: Paladin, a pseudo-phosphatase overexpressed in colon cancer, is required for actin polymerization and liver metastasis dissemination