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PXD030801

PXD030801 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAtypical U3 snoRNA Suppresses the Process of Pterygia through Modulating 18S Ribosomal RNA Synthesis
DescriptionRibosome biosynthesis plays a crucial role in regulating protein translation and is essential for cell growth and development in physiological progress. The progression and recurrence of Pterygia mainly occur due to the abnormal proliferation and migration of stromal Pterygia fibroblasts. Small nucleolar RNA U3 (U3 snoRNA), harboring the atypical C/D boxes, is involved in 18S ribosomal RNA (18S rRNA) synthesis; however, the mechanism of U3 snoRNA in Pterygia remains unclear. Methods: Primary HCFs and HPFs were separated and cultured from fresh conjunctival grafts and Pterygia tissues. The PLKO.1 lentiviral system and CRISPR/Cas9 recombinant construct were, respectively, used to overexpress and silence U3 snoRNA in HPF and HCF cells for further specific phenotypes analysis. RNA-seq and TMT-labeled quantitative protein mass spectrometry were utilized to evaluate the effect of U3 snoRNA on mRNA transcripts and protein synthesis. Results: Reduced U3 snoRNA in Pterygia promotes HCF or HPF cells' proliferation, migration, and cell cycle but has no significant effect on apoptosis. U3 snoRNA modulates 18S rRNA synthesis through shearing precursor ribosomal RNA 47S rRNA at the 5′ external transcribed spacer (5′ ETS). Moreover, the altered U3 snoRNA causes mRNA and protein differential expression in HCF or HPF cells. Conclusion: The atypical U3 snoRNA regulates the translation of specific proteins to exert a suppressive function in Pterygia through modulating the 18S RNA synthesis. Here, we uncover a novel insight into U3 snoRNA biology in the development of Pterygia.
HostingRepositoryPRIDE
AnnounceDate2022-05-20
AnnouncementXMLSubmission_2022-05-20_04:35:15.670.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitteryanzhen Zhao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-07 02:38:59ID requested
12022-05-20 04:35:16announced
Publication List
Zhang X, Jiang Y, Wang Q, An W, Zhang X, Xu M, Chen Y, Atypical U3 snoRNA Suppresses the Process of Pterygium Through Modulating 18S Ribosomal RNA Synthesis. Invest Ophthalmol Vis Sci, 63(4):17(2022) [pubmed]
Keyword List
submitter keyword: U3 snoRNA, Pterygium, Ribosome biosynthesis, 18S rRNA, 47S rRNA, 5' ETS
Contact List
xin zhang
contact affiliationDepartment of Ophthalmology, Yangpu Hospital, School of Medicine, Tongji University, Shanghai 200090, China
contact emailxinzph134@163.com
lab head
yanzhen Zhao
contact affiliationshanghai
contact email449096780@qq.com
dataset submitter
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