PXD030790
PXD030790 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Immunoprecipitation of Myc-UBL3 from MutuDC1940 cells |
| Description | Ubl3KO MutuDC or Ubl3KO MutuDC stably expressing either Myc-UBL3(WT) or Myc-UBL3(C114S) were lysed in 1% Triton buffer on ice and nuclei removed by centrifugation at 14,000 × g at 4 °C. Postnuclear supernatants of 5 x 10^6 cells were incubated with anti-Myc-Tag (9B11) mouse mAb conjugated to magnetic beads (Cell Signaling Technologies) at 4 ºC, washed and eluted by acidification with 0.1% FA and denatured with 2,2,2-trifluoroethanol. Proteins were reduced with 12.5 mM tris(2-carboxyethyl)phosphine (TCEP), and digested with 0.25 mg proteomics grade Trypsin (Sigma-Aldrich) in 50 mM triethylammonium bicarbonate (TEAB)) overnight at 37 °C. Peptides were dried by SpeedVac, reconstituted in 2% acetonitrile and 0.05% trifluoroacetic acid, centrifuged for 10 minutes at 21,000 × g to remove particulate matter, and filtered over Vivacon 500 30kDa columns (Satrorius). |
| HostingRepository | jPOST |
| AnnounceDate | 2022-01-07 |
| AnnouncementXML | Submission_2022-09-18_03:04:32.087.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Haiyin Liu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-01-06 20:45:18 | ID requested | |
| 1 | 2022-01-07 07:00:18 | announced | |
| ⏵ 2 | 2022-09-18 03:04:32 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: ubiquitin-like protein, immunoprecipitation, LFQ |
Contact List
| Justine Mintern | |
|---|---|
| lab head | |
| Haiyin Liu | |
| contact affiliation | The University of Melbourne |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001444/ |
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