PXD030771

PXD030771 is an original dataset announced via ProteomeXchange.

Title	Loss of H3K9 tri-methylation alters mitotic chromosome compaction and transcription factor retention during mitosis
Description	Recent studies have shown that repressive chromatin machinery, including DNA methyltransferases (DNMTs) and Polycomb Repressor Complexes (PRCs), bind to chromosomes throughout mitosis and their depletion results in increased chromosome size. Enzymes that catalyse H3K9 methylation, such as Suv39h1, Suv39h2, G9a, GLP are also retained by mitotic chromosomes. Surprisingly however, mutants lacking H3K9me3 have unusually small and compact mitotic chromosomes that are associated with increased H3S10ph and H3K27me3 levels. Chromosome size and centromere compaction in these mutants was rescued by providing exogenous Suv39h1, or inhibiting Ezh2 activity. Quantitative proteomic comparisons of native mitotic chromosomes isolated from wildtype versus Suv39h1,2 double null ESCs revealed that H3K9me3 was essential for the efficient retention of bookmarking factors such as Esrrb. These results highlight an unexpected role for repressive heterochromatin domains in preserving transcription factor binding through mitosis, and underscore the importance of H3K9me3 for sustaining chromosome architecture and epigenetic memory during cell division.
HostingRepository	PRIDE
AnnounceDate	2022-10-12
AnnouncementXML	Submission_2022-10-12_11:01:50.374.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HolgerKramer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-06 01:30:42	ID requested
⏵ 1	2022-10-12 11:01:50	announced

Dataset with its publication pending

submitter keyword: Chromatin

mitotic chromosomes

H3K9me3

Suv39h1

Suv39h2

Ezh2

Esrrb

HolgerKramer
contact affiliation	Proteomics & Metabolomics Facility MRC London Institute of Medical Sciences Hammersmith Hospital Campus Du Cane Road London, W12 0NN United Kingdom
contact email	h.kramer@lms.mrc.ac.uk
lab head
HolgerKramer
contact affiliation	MRC London Institute of Medical Sciences
contact email	h.kramer@lms.mrc.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030771
     1. Label: PRIDE project
     2. Name: Loss of H3K9 tri-methylation alters mitotic chromosome compaction and transcription factor retention during mitosis