PXD030768

PXD030768 is an original dataset announced via ProteomeXchange.

Title	Global crotonylatome and function analysis of lysine-crotonylated proteins in wheat response to cold stress
Description	Abstract Lysine crotonylation (Kcr) is a recently identified post-translational modification (PTM) in the prokaryotic and eukaryotic organisms. However, the function of lysine-crotonylated proteins in response to abiotic stress in plants remains largely unknown. Here, we report the first global profiling of the Kcr proteome in common wheat, identifying 4,696 Kcr sites on 1,726 substrate proteins, participating in a wide variety of biological and metabolic pathways. Combination of transcriptome and proteome in a RIL (recombinant inbred line) population, Kcr proteome, and genome-wide association study, a candidate gene phosphoglycerate kinase (TaPGK) was identified to potentially affect on cold tolerance in common wheat. Using EMS-mutants, overexpressed transgenic wheat plants and CRISPR/Cas9-mediated know-out mutants, we demonstrated that TaPGK played a positively key role in regulation of plant cold tolerance. Moreover, TaPGK protein strongly interacted with sirtuin-like (TaSRT1). EMS-mutant tetraploid and overexpressed transgenic wheat experiments confirmed that TaSRT1 gene negatively regulated wheat cold tolerance. Immunoprecipitation demonstrated that TaSRT1 promoted the degradation of TaPGK by erasing Kcr. Additionally, RNA-sequencing results showed that overexpressed TaPGK significantly improved expression of peroxidase genes. It concluded that a large number of Kcr sites were present in wheat important proteins and TaPGK crotonylated by TaSRT1 played a key role in wheat response to cold stress through scavenging the accumulation of ROS.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:36.604.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ning Zhang
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-06 01:23:33	ID requested
1	2023-07-20 10:46:18	announced
⏵ 2	2023-11-14 09:02:44	announced	2023-11-14: Updated project metadata.

Zhang N, Wang S, Zhao S, Chen D, Tian H, Li J, Zhang L, Li S, Liu L, Shi C, Yu X, Ren Y, Chen F, model regulating wheat cold tolerance through mediating pyruvate. Sci Adv, 9(19):eadg1012(2023) [pubmed]

submitter keyword: crotonylatome

Cold stress

common wheat

TaPGK

ROS

Feng Chen
contact affiliation	Agronomy College, Henan Agricultural University, China
contact email	chf0088@163.com
lab head
Ning Zhang
contact affiliation	Henan Agricultural University
contact email	zhangning88012@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030768
     1. Label: PRIDE project
     2. Name: Global crotonylatome and function analysis of lysine-crotonylated proteins in wheat response to cold stress