PXD030765

PXD030765 is an original dataset announced via ProteomeXchange.

Title	RACK1 associates with RNA-binding proteins Vigilin and SERBP1 to influence dengue virus replication
Description	Dengue virus (DENV), a re-emerging virus transmitted by Aedes mosquitoes, causes severe pathogenesis in humans. No effective treatment is available against this virus. We recently identified the scaffold protein RACK1 as a component of the DENV replication complex, a macromolecular complex essential for viral genome amplification. Here, we show that RACK1 is important for DENV infection. RACK1 mediates DENV replication through binding to the 40S ribosomal subunit. Mass spectrometry analysis of RACK1 partners coupled to a loss-of-function screen identified the RNA binding proteins Vigilin and SERBP1 as DENV host dependency factors. Vigilin and SERBP1 interact with DENV viral RNA (vRNA), forming a ternary complex with RACK1 to mediate viral replication. Overall, our results indicate that RACK1 recruits Vigilin and SERBP1, linking the DENV vRNA to the translation machinery for optimal translation and replication.
HostingRepository	PRIDE
AnnounceDate	2022-02-04
AnnouncementXML	Submission_2022-02-04_01:25:22.160.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexis Brugier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-06 01:16:33	ID requested
⏵ 1	2022-02-04 01:25:22	announced

Dataset with its publication pending

submitter keyword: RACK1, Vigilin, SERBP1, DENV, proteomics, siRNA screen, RNA binding protein, RNA stability, translation optimization, cellular factors

Ali Amara
contact affiliation	INSERM
contact email	ali.amara@inserm.fr
lab head
Alexis Brugier
contact affiliation	INSERM
contact email	alexis.brugier@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030765

PRIDE project URI

• PRIDE
  1. PXD030765
     1. Label: PRIDE project
     2. Name: RACK1 associates with RNA-binding proteins Vigilin and SERBP1 to influence dengue virus replication