PXD030755

PXD030755 is an original dataset announced via ProteomeXchange.

Title	Revisiting non-coding nature of viroids
Description	Viroids are small circular highly structured pathogens infecting a broad range of plants, caus-ing economic losses. Since their discovery in the 70s, they have been considered as non-coding pathogens. In the last few years the discovery of other RNA entities, similar in terms of size and structure, that where shown to be translated (e.g cirRNAs, precursors of miRNA, RNA sat-ellites) as well as studies showing that some viroids are located in ribosomes, have reemerged the idea that viroids may be translated. In this study we used advanced bioinformatic analy-sis, in vitro experiments and LC-MS/MS to search for small viroid peptides of the Pospiviroidae PSTVd. Our results suggest that in our experimental conditions, even though the circular form of PSTVd is found in ribosomes, no produced peptides were identified. This indicates that the presence of PSTVd in ribosomes is most probably not related to peptide pro-duction but rather to another unknown function that requires further study
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:23:47.793.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 13:10:36	ID requested
⏵ 1	2022-02-17 06:23:51	announced

Katsarou K, Adkar-Purushothama CR, Tassios E, Samiotaki M, Andronis C, Lis, ó, n P, Nikolaou C, Perreault JP, Kalantidis K, Revisiting the Non-Coding Nature of Pospiviroids. Cells, 11(2):(2022) [pubmed]

submitter keyword: Viroid, translation, non-coding RNA, cirRNA, Mass spectrometry, PSTVd

Kriton Kalantidis
contact affiliation	Department of Biology, University of Crete, Vassilika Vouton, GR-71409 Heraklion/Crete, Greece Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Greece, GR-71110 Heraklion/Crete, Greece
contact email	kriton@imbb.forth.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030755

PRIDE project URI

• PRIDE
  1. PXD030755
     1. Label: PRIDE project
     2. Name: Revisiting non-coding nature of viroids