PXD030751

PXD030751 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of prefrontal cortex from Alzheimer’s Disease patients revealed novel dysregulated proteins associated to the pathology.
Description	Alzheimer’s disease (AD) is a neurodegenerative disease and the most common cause of dementia worldwide, but the mechanisms underlying AD are far from being elucidated. Thu, the study of proteins involved in its pathogenesis would allow getting further insights into the disease and identifying new markers for early diagnosis of AD. Our aim here is to analyze protein dysregulation in AD tissues by quantitative proteomics to identify proteins associated to the disease by 10-Plex TMT (Tandem Mass Tags)-based quantitative proteomics experiments were performed using frozen tissue samples from the left prefrontal cortex of AD patients, and healthy individuals and patients with other dementias as controls. LC-MS/MS analyses were performed using a Q Exactive mass spectrometer, and a total of 3281 proteins were identified and quantified using MaxQuant. After analysis with Perseus, 31 and 250 proteins were identified as upregulated and downregulated, respectively, at two or three Braak stages with a statistically significant ≥1.5-fold expression difference in AD patients in comparison to controls. After bioinformatics analysis, 41 dysregulated proteins were selected as more prone to be associated to AD, and 10 out of them were selected to investigate their role in the pathogenesis of AD and to verify their dysregulation in the disease by qPCR, WB, immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and/or ELISA, using tissue and serum samples of AD patients, patients with other dementias and healthy individuals.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:24:53.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Montero Calle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 13:01:34	ID requested
1	2024-01-24 15:52:15	announced
⏵ 2	2024-10-22 06:24:54	announced	2024-10-22: Updated project metadata.

10.1007/S00018-023-04791-Y;

submitter keyword: Dysregulation, TMT, Proteomics,Alzheimer's disease

Rodrigo Barderas
contact affiliation	Chronic Disease Programme (UFIEC), Instituto de Salud Carlos III, Majadahonda, E-28220 Madrid, Spain.
contact email	r.barderasm@isciii.es
lab head
Ana Montero Calle
contact affiliation	Instituto de Salud Carlos III
contact email	anamonterocalle@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030751
     1. Label: PRIDE project
     2. Name: Proteomics analysis of prefrontal cortex from Alzheimer’s Disease patients revealed novel dysregulated proteins associated to the pathology.