Updated project metadata. Sideroflexins (SFXN) are evolutionarily conserved mitochondrial carriers belonging to the SCL56 family. Because little is known about SFXN1 interactome, a high-throughput search of SFXN1 binding partners was launched with the aim to better understand its mitochondrial functions. The large-scale identification of SFXN1 physical partners was based on co-immunoprecipitation followed by shotgun mass spectrometry (coIP/LC-MSMS).