PXD030733

PXD030733 is an original dataset announced via ProteomeXchange.

Title	the expression of BDNF and the levels of this neurotrophin influence the behavioural response in Zebrafish
Description	Experimental evidence suggests that environmental stress conditions can alter the expression of BDNF and the levels of this neurotrophin influence the behavioural response in mammalian models. Recently it has been demonstrated that the exposure to 34° C for 21 days alters the brain proteome and behaviour in zebrafish. The aim of this work was to investigate the role of BDNF in the nervous system of adult zebrafish under control and heat treatment conditions. For this purpose, wild type (WT), heterozygous BDNF+/- (HT) and knock out BDNF-/- (KO) zebrafish were kept for 21 days at 26° C or 34° C and then euthanized for brain analysis or subjected to behavioural tests (Y-maze test, novel tank test, light and dark test, social preference test, mirror biting test) used for assessing behavioural aspects such as boldness, anxiety, social preference, aggressivity, interest for the novel environment and exploration. RTqPCR analysis showed the reduction of gene expression of BDNF and its receptors after heat treatment. Moreover, proteomic analysis and behavioural tests showed genotype- and temperature-dependent effects on brain proteome and behaviour of zebrafish.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_07:59:20.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesca Grassi Scalvini
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 01:52:21	ID requested
⏵ 1	2022-06-09 07:59:21	announced

Maffioli E, Angiulli E, Nonnis S, Grassi Scalvini F, Negri A, Tedeschi G, Arisi I, Frabetti F, D'Aniello S, Alleva E, Cioni C, Toni M, ) Exposed to Two Different Temperatures. Int J Mol Sci, 23(10):(2022) [pubmed]

submitter keyword: proteomics, nano LC-MS/MS

Francesca Grassi Scalvini
contact affiliation	Dipartimento di medicina veterinaria
contact email	francesca.grassiscalvini@unimi.it
lab head
Francesca Grassi Scalvini
contact affiliation	Università degli studi di Milano
contact email	francesca.grassiscalvini@guest.unimi.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030733
     1. Label: PRIDE project
     2. Name: the expression of BDNF and the levels of this neurotrophin influence the behavioural response in Zebrafish