PXD030728

PXD030728 is an original dataset announced via ProteomeXchange.

Title	FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards
Description	Absolute (molar) quantification of proteins provides the analytical rationale for system-level modelling of diverse molecular mechanisms. FastCAT method employs multiple short (<50 kDa) stable-isotope labeled chimeric proteins (CPs) composed of concatenated quantotypic (Q-) peptides representing the quantified proteins. Each CP also comprises scrambled sequences of reference (R-) peptides that relate its abundance to a single protein standard (BSA). FastCAT not only alleviates the need in purifying CP or using SDS-PAGE, but also improves the accuracy, precision and dynamic range of the absolute quantifications by grouping Q-peptides according to the expected abundance of target proteins. We benchmarked FastCAT against the reference method of MS Western and tested it in the direct molar quantifications of neurological markers in human cerebrospinal fluid at the low ng/mL level.
HostingRepository	PRIDE
AnnounceDate	2022-04-07
AnnouncementXML	Submission_2022-04-07_09:14:48.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignacy Rzagalinski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-05 01:35:45	ID requested
⏵ 1	2022-04-07 09:14:48	announced

Dataset with its publication pending

submitter keyword: absolute quantification of proteins

MS Western

QconCAT

targeted quantitative proteomics

cerebrospinal fluid

neurodegeneration

neuroinflammation

Andrej Shevchenko
contact affiliation	1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
contact email	shevchenko@mpi-cbg.de
lab head
Ignacy Rzagalinski
contact affiliation	Max Planck Institute of Molecular Cell Biology and Genetics
contact email	rzagalin@mpi-cbg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030728

PRIDE project URI

• PRIDE
  1. PXD030728
     1. Label: PRIDE project
     2. Name: FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards