PXD030698

PXD030698 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of isolated A. thaliana plastoglobules from wild-type, abc1k6-1 and Comp3
Description	The quantitative proteomes of isolated plastoglobules have been determined from A. thaliana leaf chloroplasts at 4 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA insertion mutant disrupting the plastoglobule-localized ABC1K6 protein (abc1k6-1) that is delayed in transition to reproductive growth, and iii) a complementation line (Comp3) that restores the timely progression of development with the ABC1K6 wild-type protein sequence tagged C-terminally with GFP in the abc1k6-1 background. The levels of ten plastoglobule proteins were found to be effected by the loss of ABC1K6. Of particular note, ABC1K7 was strongly depleted in abc1k6-1 and over-accumulated in Comp3, suggesting it may be stabilized by ABC1K6.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:05.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Lundquist
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-04 01:09:45	ID requested
1	2022-03-02 12:00:04	announced
⏵ 2	2023-11-14 08:55:06	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: coq8,plastoglobules, adck3, abc1

Peter Lundquist
contact affiliation	Michigan State University, Department of Biochemistry & Molecular Biology
contact email	pklundqu@msu.edu
lab head
Peter Lundquist
contact affiliation	Michigan State University
contact email	PKLUNDQU@MSU.EDU
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030698
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of isolated A. thaliana plastoglobules from wild-type, abc1k6-1 and Comp3