PXD030695

PXD030695 is an original dataset announced via ProteomeXchange.

Title	Ion-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions
Description	Unbiased drug target engagement deconvolution and mechanism of action elucidation are major challenges in drug development. Modification-free target engagement methods, such as thermal proteome profiling, have gained increasing popularity over the last several years. However, these methods have limitations, and, in any case, new orthogonal approaches are needed. Here we present a novel isothermal method for comprehensive characterization of protein solubility alterations using the effect on protein solubility of cations and anions in the Hofmeister series. We combine this ion-based protein precipitation approach with Proteome Integrated Solubility Alteration (PISA) analysis and use this I-PISA assay to delineate the targets of several anti-cancer drugs both in cell lysate and in living cells. Lastly, we demonstrate that I-PISA can detect solubility changes in minute amounts of sample, opening chemical proteomics applications to small and rare biological material.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_07:50:42.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Beusch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-04 01:02:40	ID requested
⏵ 1	2022-06-09 07:50:43	announced

Beusch CM, Sabatier P, Zubarev RA, Ion-Based Proteome-Integrated Solubility Alteration Assays for Systemwide Profiling of Protein-Molecule Interactions. Anal Chem, 94(19):7066-7074(2022) [pubmed]

submitter keyword: Cancer, drug target deconvolution, mechanism of action, proteomics, thermal protein profiling

Roman Zubarev
contact affiliation	Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm 17177, Sweden
contact email	roman.zubarev@ki.se
lab head
Christian Beusch
contact affiliation	Division of Chemistry I Department of Medical Biochemistry & Biophysics Karolinska Institutet
contact email	christian.beusch@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD030695

PRIDE project URI

• PRIDE
  1. PXD030695
     1. Label: PRIDE project
     2. Name: Ion-based Proteome Integrated Solubility Alteration Assay for System-wide Profiling of Protein-Molecule Interactions