PXD030679

PXD030679 is an original dataset announced via ProteomeXchange.

Title	Characterization of novel human cystatin B salivary interactome in Alzheimer’s disease and healthy subjects.
Description	Alzheimer’s disease (AD) is the most prevalent neurodegenerative disease in the elderly, characterized by the accumulation in the brain of misfolded proteins, inflammation, and oxidative damage leading to neuronal cell death. The necessity of searching for new non-invasive biomarkers in tissues or body fluids easy and painless to collect has been evidenced. In this context, saliva has been chosen to investigate new possible biomarkers using a proteomics-based approach. Previous results obtained on the acid-soluble fraction of saliva from AD patients by a Top-Down proteomic approach revealed the potential role of cystatin B in the disease, a protein capable of interacting with other proteins, including amyloid beta, and to homo-polymerize. Thus, the first aim of the present project has been to demonstrate the existence of a multiprotein complex with cystatin B in whole saliva samples and to characterize it, secondly, we further studied the cystatin B interactome to individuate any qualitative-quantitative difference between AD patients and healthy subjects age and sex matched. To these aims, we performed some preliminary tests mainly by western blot to look for high molecular weight positive signals related to cystatin B in saliva, and, then, in order to characterize the presence of potential multiprotein complexes, we performed Co-IP assays followed by in gel tryptic digestion and RP-nanoHPLC-HR-ESI-MS/MS analysis (reverse-phase nano-high-performance liquid chromatography separation coupled to electrospray-ion trap tandem mass spectrometry).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:05.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristina Contini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); L-methionine removal; O-phospho-L-threonine; mono N-acetylated residue; monohydroxylated residue; O-phospho-L-serine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-31 02:06:13	ID requested
1	2023-05-10 16:30:00	announced
⏵ 2	2023-11-14 08:48:07	announced	2023-11-14: Updated project metadata.

Contini C, Serrao S, Manconi B, Olianas A, Iavarone F, Guadalupi G, Messana I, Castagnola M, Masullo C, Bizzarro A, Turck CW, Maccarrone G, Cabras T, Characterization of Cystatin B Interactome in Saliva from Healthy Elderly and Alzheimer's Disease Patients. Life (Basel), 13(3):(2023) [pubmed]

submitter keyword: CoIP, proteomics, Alzheimer, cystatinB,LC-MSMS, interactome

Tiziana Cabras
contact affiliation	Department of Life and Environmental Sciences, University of Cagliari
contact email	tcabras@unica.it
lab head
Cristina Contini
contact affiliation	University of Cagliari
contact email	c.contini@unica.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030679
     1. Label: PRIDE project
     2. Name: Characterization of novel human cystatin B salivary interactome in Alzheimer’s disease and healthy subjects.