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PXD030671

PXD030671 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatial Proteomic Analysis of Isogenic Metastatic Colorectal Cancer Cells Reveals Key Dysregulated Proteins Associated with Lymph Node, Liver and Lung Metastasis
DescriptionLiver, lung and lymph nodes are the most common metatastic sites of colorectal cancer (CRC) cells. Here, we aimed to analyze by quantitative spatial proteomics the isogenic KM12 cell system (non-metastatic KM12C cells, liver metastatic KM12SM cells and liver and lung metastatic KM12L4a cells), and the isogenic non-metastatic SW480 and lymph nodes metastatic SW620 cells to study CRC metastasis. Cells were fractionated to study by proteomics five subcellular fractions corresponding to cytoplasm (CEB), membrane (MEB), nucleus (NEB), chromatin-bound proteins (NEB-CBP), and cytoskeletal proteins (PEB), and the secretome. Protein extracts were trypsin digested, labeled with TMT 11-plex and fractionated prior to proteomics analysis on a Q Exactive. We provide data on protein abundance and localization of 4031 proteins in their different subcellular fractions, depicting dysregulation of proteins in abundance and/or localization in the most common sites of CRC metastasis. Alterations in abundance and localization for selected proteins were validated via WB, IF, IHC and ELISA using CRC cells and patients’ tissue and plasma samples. These results supported the relevance of the proteomics results in a real-life scenario of CRC metastasis.
HostingRepositoryPRIDE
AnnounceDate2022-02-22
AnnouncementXMLSubmission_2022-02-22_04:23:39.836.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAna Montero Calle
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-30 03:49:14ID requested
12022-02-22 04:23:40announced
Publication List
Sol, í, s-Fern, á, ndez G, Montero-Calle A, Mart, í, nez-Useros J, L, ó, pez-Janeiro Á, de Los R, í, os V, Sanz R, Dziakova J, Milagrosa E, Fern, á, ndez-Ace, ñ, ero MJ, Pel, á, ez-Garc, í, a A, Casal JI, Hofkens J, Rocha S, Barderas R, Spatial Proteomic Analysis of Isogenic Metastatic Colorectal Cancer Cells Reveals Key Dysregulated Proteins Associated with Lymph Node, Liver, and Lung Metastasis. Cells, 11(3):(2022) [pubmed]
Keyword List
submitter keyword: Colorectal cancer, Cells, TMT 11-Plex, Spatial Proteomics
Contact List
Rodrigo Barderas
contact affiliationChronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
contact emailr.barderasm@isciii.es
lab head
Ana Montero Calle
contact affiliationInstituto de Salud Carlos III
contact emailanamonterocalle@hotmail.com
dataset submitter
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Dataset FTP location
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