PXD030670

PXD030670 is an original dataset announced via ProteomeXchange.

Title	Hydrophilic interaction liquid chromatography contributes to integrated N-glycoproteomics analysis of human saliva for lung cancer
Description	We aimed to in-depth characterize and quantify salivary N-glycoproteomics. HILIC enrichment and LC-MS/MS were combined to investigate salivary glycosylation both at deglycopeptides level and glycopepeptides level, and alterations in site-specific glycoforms for human saliva were detected between lung cancer patients and healthy subjects. Firstly, intact glycopeptides were enriched from human saliva through using HILIC. Obtained intact glycopeptides were characterized by LC-MS/MS directly. Furthermore, the glycosylation sites were fully identified by LC-MS/MS followed by incubating with PNGase F. The developed workflow was applied to compare N-glycosites and intact N-glycopeptides in lung cancer group and healthy control group. Dysregulated N-glycosites as well as site-specific glycoforms were confidently observed in lung cancer and their potential value in clinical applications will be discussed.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:28.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiyu Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-30 03:47:35	ID requested
1	2023-02-08 01:17:22	announced
⏵ 2	2023-11-14 08:54:29	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Cancer (B/D-HPP), Human Proteome Project

submitter keyword: HILIC, Lung cancer, N-glycosites,Saliva, Intact N-glycopeptides

Hua Xiao
contact affiliation	State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
contact email	huaxiao@sjtu.edu.cn
lab head
Huiyu Wang
contact affiliation	18272583259
contact email	lizlemon@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030670
     1. Label: PRIDE project
     2. Name: Hydrophilic interaction liquid chromatography contributes to integrated N-glycoproteomics analysis of human saliva for lung cancer