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PXD030664

PXD030664 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of Masson pine with high resistance to pine wood nematode
DescriptionPine wilt disease is a worldwide dangerous pine disease. We used Masson pine (Pinus massoniana) clones, selected through traditional breeding and testing for 20 years, with high resistance to study the molecular mechanism of resistance to pine wood nematode (PWN, Bursaphelenchus xylophilus). A total of 3491 proteins were identified from seedling tissue, among which 2783 proteins contained quantitative information. Total 42 proteins were up-regulated and 96 proteins were down-regulated in resistant lines. Of them, function enrichment analysis found that significant differences in proteins with pectin esterase activity or peroxidase activity. Proteins participating in salicylic acid metabolism, antioxidant stress reaction, polysaccharide degradation, glucose acid ester sheath lipid biosynthesis, sugar glycosaminoglycans degradation pathway also changed significantly. PRM results showed that pectin acetyl esterase, carbonic anhydrase, peroxidase and chitinase were significantly down-regulated, while aspartic protease was significantly up-regulated, which was consistent with proteomic data.These results suggested that Masson pine could degrade nematode-related proteins by increasing protease to inhibit their infestation, and enhance the resistance of Masson pine to PWN by down-regulating the carbon metabolism to limit available carbon to PWN or to be involved in cell wall components or tissue softening. Most downregulated proteins seem to take back seats prior to pathogen attacks. The highly resistant Masson pine, very likely, has evolved multiple pathways, both the passive and active, to defense against PWN infestation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:17:33.181.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJingbin Gao
SpeciesList scientific name: Pinus massoniana var. massoniana; NCBI TaxID: 714485;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-30 03:31:45ID requested
12022-10-13 14:26:33announced
22023-11-14 07:17:34announced2023-11-14: Updated project metadata.
Publication List
Gao J, Pan T, Chen X, Wei Q, Xu L, Proteomic analysis of Masson pine with high resistance to pine wood nematodes. PLoS One, 17(8):e0273010(2022) [pubmed]
Keyword List
submitter keyword: Masson pine
pine wood nematode
resistant proteins
proteomic analysis
Contact List
Jingbin Gao
contact affiliationState Key Laboratory of the National Forestry and Grassland Administration for Pine Wood Nematode Disease Prevention and Control Technology, Anhui Academy of Forestry, Hefei 230001, China
contact emailjingbingao@163.com
lab head
Jingbin Gao
contact affiliationAnhui Vocational and Technical College of Forestry
contact emailjingbingao@163.com
dataset submitter
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