PXD030623 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores |
Description | Kinetochores assemble on centromeres (CENs) via histone H3 variant CENP-A and low levels of CEN transcripts. RNA polymerase II (RNAPII) activity is restrained by the CEN histone code, while CEN RNA concentrations are reduced by the nuclear exosome. Using S. cerevisiae, we add kinase Rio1 to this scheme as it downregulates RNAPII, and promotes CEN RNA turnover via exoribonuclease Rat1. Transcription factor Cbf1, and the assembled kinetochore further restrain CEN transcription. CEN transcripts exist as long (up to 11,000nt) and short RNAs (119±40nt), which may underlie CEN identity and kinetochore recruitment. While also curtailed by Rio1, Rat1, and the exosome, periCEN RNAs (<200nt) accumulate at levels that are one order of magnitude higher than the CEN transcripts. Depleting Rio1 causes CEN and periCEN RNA buildup, kinetochore malformation, and chromosome loss. Depleting human orthologue RioK1 leads to CEN RNA accumulation and micronuclei formation, suggesting that Rio1/RioK1 activity at centromeres is conserved. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-24 |
AnnouncementXML | Submission_2025-02-24_00:44:31.992.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD030623 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Romina Belli |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-23 03:03:17 | ID requested | |
⏵ 1 | 2025-02-24 00:44:32 | announced | |
Publication List
Keyword List
submitter keyword: Rat1, kinetochore, centromere, RioK1, pericentromere,Rio1 |
Contact List
Peter De Wulf |
contact affiliation | University of Trento - Chromosome Segregation Lab - CIBIO |
contact email | peter.dewulf@unitn.it |
lab head | |
Romina Belli |
contact affiliation | University of Trento |
contact email | romina.belli@unitn.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030623
- Label: PRIDE project
- Name: Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores