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PXD030623

PXD030623 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores
DescriptionKinetochores assemble on centromeres (CENs) via histone H3 variant CENP-A and low levels of CEN transcripts. RNA polymerase II (RNAPII) activity is restrained by the CEN histone code, while CEN RNA concentrations are reduced by the nuclear exosome. Using S. cerevisiae, we add kinase Rio1 to this scheme as it downregulates RNAPII, and promotes CEN RNA turnover via exoribonuclease Rat1. Transcription factor Cbf1, and the assembled kinetochore further restrain CEN transcription. CEN transcripts exist as long (up to 11,000nt) and short RNAs (119±40nt), which may underlie CEN identity and kinetochore recruitment. While also curtailed by Rio1, Rat1, and the exosome, periCEN RNAs (<200nt) accumulate at levels that are one order of magnitude higher than the CEN transcripts. Depleting Rio1 causes CEN and periCEN RNA buildup, kinetochore malformation, and chromosome loss. Depleting human orthologue RioK1 leads to CEN RNA accumulation and micronuclei formation, suggesting that Rio1/RioK1 activity at centromeres is conserved.
HostingRepositoryPRIDE
AnnounceDate2025-02-24
AnnouncementXMLSubmission_2025-02-24_00:44:31.992.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030623
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRomina Belli
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-23 03:03:17ID requested
12025-02-24 00:44:32announced
Publication List
10.6019/PXD030623;
Keyword List
submitter keyword: Rat1, kinetochore, centromere, RioK1, pericentromere,Rio1
Contact List
Peter De Wulf
contact affiliationUniversity of Trento - Chromosome Segregation Lab - CIBIO
contact emailpeter.dewulf@unitn.it
lab head
Romina Belli
contact affiliationUniversity of Trento
contact emailromina.belli@unitn.it
dataset submitter
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Dataset FTP location
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