PXD030619

PXD030619 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry Uncovers Protein Interactions and Stoichiometry of Intermediates and Sub-complexes for SNARE Complex Assembly
Description	The neuronal SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 anchored to the presynaptic membrane. It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters into the synaptic cleft. While the general sequence of SNARE complex formation is well-established, our knowledge on possible intermediates is still incomplete. We, therefore, follow the step-wise assembly of the SNARE complex and target individual SNAREs, binary sub-complexes, the ternary SNARE complex as well as interactions with Complexin-1. Using native mass spectrometry, we identify the stoichiometry of preferred sub-complexes and monitor oligomerisation of various assemblies. Importantly, we find that interactions with Complexin-1 reduce self-association of the ternary SNARE complex. Chemical cross-linking provides detailed insights into these interactions suggesting a role during membrane fusion. Taken together, we unravel possible intermediates and preferred sub-complexes and compile a road map of SNARE complex assembly including regulation by Complexin-1.
HostingRepository	PRIDE
AnnounceDate	2023-01-25
AnnouncementXML	Submission_2023-01-25_11:38:19.293.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MarieAlfes
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-23 00:34:28	ID requested
⏵ 1	2023-01-25 11:38:19	announced

Dataset with its publication pending

submitter keyword: native mass spectrometry, chemical cross-linking,SNARE complex, Complexin-1, signal transmission in neurons

CarlaSchmidt
contact affiliation	Interdisciplinary Research Centre HALOmem, Charles Tanford Protein Centre, Institute of Biochemistry and Biotechnology, Martin-Luther University Halle-Wittenberg, Halle, Germany
contact email	carla.schmidt@biochemtech.uni-halle.de
lab head
MarieAlfes
contact affiliation	HALOmem, Martin Luther University Halle-Wittenberg
contact email	marie.barth@bct.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030619
     1. Label: PRIDE project
     2. Name: Mass Spectrometry Uncovers Protein Interactions and Stoichiometry of Intermediates and Sub-complexes for SNARE Complex Assembly