PXD030618

PXD030618 is an original dataset announced via ProteomeXchange.

Title	Activity-based protein profiling assay for functional biocatalyst screening in white rot fungus Phanerochaete chrysosporium grown on beech wood chips
Description	Activity-based protein profiling (ABPP) has emerged as a versatile biochemical method for studying enzyme activity under various physiological conditions, with applications mainly in the field of biomedicine. Here, we show the potential of ABPP in the discovery of biocatalysts from Phanerochaete chrysosporium suspension cultures grown on beech wood chips. P. chrysosporium is a thermophilic white rot fungus that expresses a large enzyme repertoire for efficient lignocellulose hydrolysis. By employing a comparative ABPP approach, we could preselect lignocellulose-degrading serine and glycoside hydrolases from this enzyme repertoire for further biochemical characterization. In addition to the supernatant, also a substrate-bound protein fraction isolated from wood chips was screened for active enzymes. As heterologous expression of fungal enzymes remains challenging, our ABPP-mediated preselection procedure allows to focus experimental efforts on the most promising biocatalysts. Besides, we show that this approach can also be used to functionally annotate domains-of-unknown functions (DUFs). ABPP-based biocatalyst screening may thus allow the elucidation of biocatalysts with novel gene sequences, and we anticipate that the presented ABPP approach will find wider applications both in the discovery of novel biocatalysts and in revealing the catalytic activity of hitherto unknown enzymes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:40:20.529.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Phanerochaete chrysosporium RP-78; NCBI TaxID: 273507;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-23 00:32:18	ID requested
1	2023-03-11 02:00:07	announced
⏵ 2	2023-11-14 08:40:24	announced	2023-11-14: Updated project metadata.

Schmerling C, Sewald L, Heilmann G, Witfeld F, Begerow D, Jensen K, Br, ä, sen C, Kaschani F, Overkleeft HS, Siebers B, Kaiser M, Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling. Commun Biol, 5(1):1254(2022) [pubmed]

submitter keyword: green chemistry,Activity-based protein profiling, biocatalysis, lignocellulose degradation, carbohydrate

Farnusch Kaschani
contact affiliation	Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030618
     1. Label: PRIDE project
     2. Name: Activity-based protein profiling assay for functional biocatalyst screening in white rot fungus Phanerochaete chrysosporium grown on beech wood chips