PXD030597

PXD030597 is an original dataset announced via ProteomeXchange.

Title	Growth factor-induced alterations of plasma membrane-associated proteome of DAOY cell
Description	In this study we investigated alterations in the plasma membrane (PM)-associated proteome of DAOY medulloblastoma cells. We used a surface biotinylation approach to label PM-associated proteins. We enriched for biotinylated proteins by streptavidin-pull-down and determined the composition by mass-spectrometry. We compared CRISPR/CAS9 control cells and CRISPR/CAS9 MAP4K4 (MAP4K4 depletion) cells under starvation and after 30 min stimulation with 20 ng/ml HGF. We performed two group analyses of all conditions.
HostingRepository	PRIDE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_03:30:45.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Baumgartner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-22 02:40:50	ID requested
⏵ 1	2022-03-14 03:30:45	announced

Dataset with its publication pending

submitter keyword: Medulloblastoma, Hepatocyte growth factor, MAP4K4, Plasma membrane proteome

Martin Baumgartner
contact affiliation	Balgrist Campus Lengghalde 5 CH-8008 Zürich Switzerland
contact email	Martin.Baumgartner@kispi.uzh.ch
lab head
Martin Baumgartner
contact affiliation	University Children's Hospital Zürich
contact email	Martin.Baumgartner@kispi.uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030597
     1. Label: PRIDE project
     2. Name: Growth factor-induced alterations of plasma membrane-associated proteome of DAOY cell