PXD030595

PXD030595 is an original dataset announced via ProteomeXchange.

Title	Using mass spectrometry-based proteomics to identify candidate blood-based markers of cardiomyopathy in the southern sea otter (Enhydra lutris nereis)
Description	Cardiomyopathy is a heart muscle disease and diagnosis relies on radiography and echocardiography, while blood-based markers are lacking. Development of a non-invasive test would be useful when imaging is not possible (e.g., prenatal diagnosis). In southern sea otters (Enhydra lutris nereis), cardiomyopathy is a prevalent cause of mortality and antemortem diagnosis is challenging. Sea otters requiring clinical care are at significant anesthetic risk if cardiomyopathy is present. A blood-based assay would improve triage decisions, case management, and treatment protocols to safeguard against co-morbidities. With support from the Oiled Wildlife Care Network, we analyzed undepleted sea otter serum using mass spectrometry-based proteomics. Though the larger sample set included 63 sera (that included a validation set of 22 sera with class labels blinded to the data collector), we a priori compared only samples from wild otters. Additionally, we generated proteomic data for four heat tissues with paired sera. These results demonstrate the utility of proteomic analysis, offer a glimpse into the sea otter proteome, and serve as a reference data set for relative protein abundance in sera and cardiac tissue.
HostingRepository	PRIDE
AnnounceDate	2021-12-22
AnnouncementXML	Submission_2021-12-22_05:10:30.459.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Neely
SpeciesList	scientific name: Enhydra lutris nereis; NCBI TaxID: 1049777;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-22 02:37:53	ID requested
⏵ 1	2021-12-22 05:10:30	announced

Dataset with its publication pending

submitter keyword: sea otter, serum, LC-MS/MS

Benjamin Adam Neely
contact affiliation	National Institute of Standards and Technology
contact email	benjamin.neely@nist.gov
lab head
Benjamin Neely
contact affiliation	National Institute of Standards and Technology
contact email	benjamin.neely@nist.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030595
     1. Label: PRIDE project
     2. Name: Using mass spectrometry-based proteomics to identify candidate blood-based markers of cardiomyopathy in the southern sea otter (Enhydra lutris nereis)