PXD030582

PXD030582 is an original dataset announced via ProteomeXchange.

Title	Proteome / pYome of LYN deficient HS-5 stroma cells
Description	LYN kinase is a tyrosine kinase, that regulates cellular homeostasis in a context specific manner. Our group could show, that its expression in the leukemic microenvironment of chronic lymphocytic leukemia contributes to disease progression (Nguyen PH et al.; Cancer Cell; 2016). To analyze the effect of LYN kinase on the leukemia supportive phenotype of the bone marrow stromal cell line HS-5, we generated single cell clones of LYN deficient cells. These cells were analyzed in a Multi-Omic approach, including quantitative, label-free proteomic analysis of the Proteome / SILAC labelled analysis of the tyrosine phosphoproteome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:22.783.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander F. vom Stein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-21 12:37:51	ID requested
1	2023-05-10 13:17:19	announced
⏵ 2	2023-11-14 08:46:24	announced	2023-11-14: Updated project metadata.

Vom Stein AF, Rebollido-Rios R, Lukas A, Koch M, von Lom A, Reinartz S, Bachurski D, Rose F, Bozek K, Abdallah AT, Kohlhas V, Saggau J, Z, ö, lzer R, Zhao Y, Bruns C, Br, ö, ckelmann PJ, Lohneis P, B, ü, ttner R, H, ä, upl B, Oellerich T, Nguyen PH, Hallek M, LYN kinase programs stromal fibroblasts to facilitate leukemic survival via regulation of c-JUN and THBS1. Nat Commun, 14(1):1330(2023) [pubmed]

submitter keyword: LYN kinase, HS-5,Stroma, BMSC, Secretome, Multi-Omics

Prof. Michael Hallek
contact affiliation	University of Cologne Faculty of Medicine and Cologne University Hospital Department I of Internal Medicine Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf Center for Molecular Medicine Cologne CECAD Center of Excellence on Cellular Stress Responsesin Aging-Associated Diseases 50931 Cologne Germany
contact email	michael.hallek@uni-koeln.de
lab head
Alexander F. vom Stein
contact affiliation	University of Cologne Department I for Interal Medicine
contact email	alexander.vom-stein@uk-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD030582

PRIDE project URI

• PRIDE
  1. PXD030582
     1. Label: PRIDE project
     2. Name: Proteome / pYome of LYN deficient HS-5 stroma cells