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PXD030565

PXD030565 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChanges in endothelial cell secretome after sirtuin-1 knock-down
DescriptionSirtuin1 (Sirt1) in skeletal muscle (SK) and fat protects against metabolic damage by stimulating insulin sensitivity. Here we report that mice with selective deletion of endothelial Sirt1 (E-Sirt1-KO) paradoxically exhibit heightened whole-body insulin sensitivity. Akt phosphorylation, glucose uptake, and glycolysis are boosted in SK and brown adipose tissue (BAT) of E-Sirt1-KO mice. E-Sirt1-KO mice have higher energy expenditure and are partially protected from high-fat diet-induced insulin resistance. Enhanced insulin sensitivity and peripheral tissue Akt phosphorylation in E-Sirt1-KO mice is transferrable to wild-type mice via the systemic circulation after surgical parabiosis. Silencing of Sirt1 in endothelial cells upregulates transcription of the F-actin-binding protein thymosin beta-4 (Tβ4), whose secretion activates Akt in skeletal myotubes. Sirt1 downregulation stimulates endothelial Tβ4 transcription through inhibition of autophagy and upregulation of nuclear factor-kappa B signaling. Thus, unlike Sirt1 in skeletal muscle and fat, endothelial Sirt1 curtails whole-body insulin sensitivity by inhibiting expression of secreted Tβ4.
HostingRepositoryPRIDE
AnnounceDate2023-08-07
AnnouncementXMLSubmission_2023-08-06_18:05:16.446.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarkusBachschmid
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-21 03:18:10ID requested
12023-08-06 18:05:16announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SirT1,HUVEC, Secretome
Contact List
KailkobadIrani
contact affiliationDivision of Cardiovascular Medicine, Department of Medicine, Abboud Cardiovascular Research Center, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA. Fraternal Order of Eagles Diabetes Research Center, Pappajohn Biomedical Institute, and Heart and Vascular Center, University of Iowa, University of Iowa, Iowa City, Iowa, USA.
contact emailkaikobad-irani@uiowa.edu
lab head
MarkusBachschmid
contact affiliationQuintara Biosciences
contact emailbachschmid.markus@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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