PXD030559

PXD030559 is an original dataset announced via ProteomeXchange.

Title	Swine Acute Diarrhea Syndrome Coronavirus Induces Autophagy to Promote Its Replication via the Akt/mTOR Pathway
Description	Swine acute diarrhea syndrome coronavirus (SADS-CoV) is an enveloped, single‐stranded, positive‐sense RNA viruse belonging to Coronaviridae family. An increasing number of studies have demonstrated that viruses could utilize autophagy to promote their own replication. However, the relationship between SADS-CoV and autophagy machinery remained unknown. Here, we reported that SADS-CoV infection induced autophagy in cultured cells and pharmacologically increased autophagy was conducive to viral proliferation. Conversely, suppression of autophagy by pharmacological inhibitor or knockdown of autophagy-related protein impeded viral replication. Furthermore, we demonstrated the underlying mechanisms by which SADS-CoV triggered autophagy through inactivation of the Akt/mTOR pathway. Importantly, we identified integrin α3 (ITGA3) as a potential antiviral target upstream of Akt/mTOR and autophagy pathways by analyzing the proteomic profiles. Knockdown of ITGA3 using RNA inference activated autophagy and as a consequence, increased the replication of SADS-CoV. Collectively, our studies revealed a novel mechanism that SADS-CoV induced autophagy to facilitate its proliferation via Akt/mTOR pathway and found that ITGA3 is an effective antiviral factor for suppressing viral infection.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:47.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YU XIA
SpeciesList	scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-21 02:55:35	ID requested
1	2023-03-11 01:08:16	announced
⏵ 2	2023-11-14 08:39:48	announced	2023-11-14: Updated project metadata.

Zeng S, Zhao Y, Peng O, Xia Y, Xu Q, Li H, Xue C, Cao Y, Zhang H, Swine acute diarrhea syndrome coronavirus induces autophagy to promote its replication via the Akt/mTOR pathway. iScience, 25(11):105394(2022) [pubmed]

submitter keyword: SARS-CoV；Autophagy；ITGA3

Yongchang Cao
contact affiliation	School of Life Sience, Sun Yat-sen University
contact email	caoych@mail.sysu.edu.cn
lab head
YU XIA
contact affiliation	Sun Yat-sen University
contact email	xiay86@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030559

PRIDE project URI

• PRIDE
  1. PXD030559
     1. Label: PRIDE project
     2. Name: Swine Acute Diarrhea Syndrome Coronavirus Induces Autophagy to Promote Its Replication via the Akt/mTOR Pathway