PXD030552

PXD030552 is an original dataset announced via ProteomeXchange.

Title	Characterization of progressive denaturation and unfolding intermediates of proteins by fast cross-linking using di-ortho-phthalaldehyde
Description	Chemical cross-linking of proteins coupled with mass spectrometry (CXMS) is widely used in protein structural analysis. In this study we develop a new class of non-hydrolyzable amine-selective di-ortho-phthalaldehyde (DOPA) cross-linkers, one of which is DOPA2. Cross-linking of proteins with DOPA2 is 60-120 times faster than that with the N-hydroxysuccinimide ester cross-linker DSS. Compared with DSS cross-links, DOPA2 cross-links have a higher degree of agreement with the crystal structures of tested proteins. More importantly, DOPA2 has unique advantages of working at low pH, low temperature, or in the presence of denaturants such as 8 M urea or 6 M guanidine hydrochloride. Using staphylococcal nuclease, bovine serum albumin, and bovine pancreatic ribonuclease A, we demonstrate that DOPA2 cross-linking provides abundant spatial information about the conformations of proteins denatured to varying degrees. Lastly, we show that CXMS with only 10 seconds of DOPA cross-linking uncovers conformational changes ass
HostingRepository	iProX
AnnounceDate	2021-12-20
AnnouncementXML	Submission_2022-03-20_20:52:47.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian-Hua Wang
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-20 20:03:36	ID requested
1	2021-12-20 20:04:03	announced
⏵ 2	2022-03-20 20:52:48	announced	2022-03-21: Update publication information.

Wang JH, Tang YL, Gong Z, Jain R, Xiao F, Zhou Y, Tan D, Li Q, Huang N, Liu SQ, Ye K, Tang C, Dong MQ, Lei X, Characterization of protein unfolding by fast cross-linking mass spectrometry using di-ortho-phthalaldehyde cross-linkers. Nat Commun, 13(1):1468(2022) [pubmed]

submitter keyword: CXMS, Amine-selective cross-linker, Protein unfolding, LC-MS/MS

Meng-Qiu Dong
contact affiliation	National Institute of Biological Sciences, Beijing
contact email	dongmengqiu@nibs.ac.cn
lab head
Jian-Hua Wang
contact affiliation	National Institute of Biological Sciences, Beijing
contact email	wangjianhua@nibs.ac.cn
dataset submitter

iProX dataset URI