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PXD030551

PXD030551 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMutant KRAS-associated proteome is mainly controled by exogenous factors
DescriptionKRAS signaling has been extensively studied, yet the clarification between KRAS-autonomous and non-autonomous mechanisms are still less explored. Understanding how KRAS signaling and effects are affected by exogenous stimuli can provide valuable insights not only to understand resistance mechanisms that justify pathway inhibition failure, but also to uncover novel therapeutic targets for mutant KRAS patients. Hence, aiming at understanding KRAS-autonomous versus non autonomous mechanisms, we studied the response of two mutant KRAS colorectal cancer cell lines (HCT116 and LS174T) - control and KRAS silenced- to TGFβ1-activated fibroblasts secretome. By performing a total proteome analysis, we observed that TGFβ1-activated fibroblast-secreted factors triggered cell-line specific proteome alterations and that mutant KRAS governs approximately 1/3 of those alterations. Moreover, the analysis of the impact of exogenous factors on the modulation of KRAS proteome revealed that more than 2/3 of the KRAS-associated proteome is controlled in a KRAS-non-autonomous manner, dependent on the exogeneous factors, in both cell lines. This work highlights the context-dependency of KRAS-associated signaling and reinforces the importance of establishing more integrative models resembling the complexity of the tumor microenvironment to study KRAS-associated signals.
HostingRepositoryPRIDE
AnnounceDate2022-04-06
AnnouncementXMLSubmission_2022-04-06_09:23:47.318.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030551
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHugo Osorio
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-20 11:22:21ID requested
12022-04-06 09:23:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mutant KRAS
Colorectal cancer
Cancer associated-fibroblasts
Proteomic analysis
Contact List
Sérgia Velho
contact affiliationi3S - Instituto de Investigação e Inovação em Saúde IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto
contact emailsvelho@ipatimup.pt
lab head
Hugo Osorio
contact affiliationi3S - Institute for Research and Innovation in Health, University of Porto
contact emailhosorio@i3s.up.pt
dataset submitter
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Dataset FTP location
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