PXD030551 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mutant KRAS-associated proteome is mainly controled by exogenous factors |
Description | KRAS signaling has been extensively studied, yet the clarification between KRAS-autonomous and non-autonomous mechanisms are still less explored. Understanding how KRAS signaling and effects are affected by exogenous stimuli can provide valuable insights not only to understand resistance mechanisms that justify pathway inhibition failure, but also to uncover novel therapeutic targets for mutant KRAS patients. Hence, aiming at understanding KRAS-autonomous versus non autonomous mechanisms, we studied the response of two mutant KRAS colorectal cancer cell lines (HCT116 and LS174T) - control and KRAS silenced- to TGFβ1-activated fibroblasts secretome. By performing a total proteome analysis, we observed that TGFβ1-activated fibroblast-secreted factors triggered cell-line specific proteome alterations and that mutant KRAS governs approximately 1/3 of those alterations. Moreover, the analysis of the impact of exogenous factors on the modulation of KRAS proteome revealed that more than 2/3 of the KRAS-associated proteome is controlled in a KRAS-non-autonomous manner, dependent on the exogeneous factors, in both cell lines. This work highlights the context-dependency of KRAS-associated signaling and reinforces the importance of establishing more integrative models resembling the complexity of the tumor microenvironment to study KRAS-associated signals. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-06 |
AnnouncementXML | Submission_2022-04-06_09:23:47.318.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD030551 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hugo Osorio |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-20 11:22:21 | ID requested | |
⏵ 1 | 2022-04-06 09:23:47 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mutant KRAS |
Colorectal cancer |
Cancer associated-fibroblasts |
Proteomic analysis |
Contact List
Sérgia Velho |
contact affiliation | i3S - Instituto de Investigação e Inovação em Saúde IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto |
contact email | svelho@ipatimup.pt |
lab head | |
Hugo Osorio |
contact affiliation | i3S - Institute for Research and Innovation in Health, University of Porto |
contact email | hosorio@i3s.up.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030551
- Label: PRIDE project
- Name: Mutant KRAS-associated proteome is mainly controled by exogenous factors