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PXD030544

PXD030544 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRole of H/ACA snoRNA in translation
DescriptionTrypanosomes are unicellular parasites that cycle between the insect and mammalian host and is the causative agent of African sleeping sickness and the wasting disease, nagana, in cattle. Previous studies from our lab suggested that individual rRNA modification such as pseudouridine (Y) and 2’-O-methylation are developmentally regulated during the complex life cycle of these parasites. In this study we identified that the Y composition of translating ribosomes are different from rRNA derived from total RNA. Ablation of single snoRNA guiding Y in one of the inter-subunit bridges of the ribosome affects its affinity to translate specific mRNA. Mutational analysis in the pseudouridylation pocket of this snoRNA supports for the notion that the guided modification and not the chaperonic activity, is essential for the observed phenotype. Structural probing of rRNA using DMS-seq suggested that a single modification can affect the rRNA structure locally as wells as globally. Quantitative profiling of the composition of mutant ribosomes suggested the absence of eS12 ribosomal protein in the mature ribosomes. Addback experiments validated the role of eS12 during the loss of a single Y in these mutant cells. Orthologous experiments in related leishmania spp. also verify importance of a single Y in the function of ribosome. Ongoing experiments intend to visualize such ribosomes lacking a single RNA modification and decipher their significance towards ribosome structure.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:25:33.932.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTirza Doniger
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-20 11:04:59ID requested
12024-01-26 06:50:09announced
22024-10-22 06:25:37announced2024-10-22: Updated project metadata.
Publication List
Rajan KS, Madmoni H, Bashan A, Taoka M, Aryal S, Nobe Y, Doniger T, Galili Kostin B, Blumberg A, Cohen-Chalamish S, Schwartz S, Rivalta A, Zimmerman E, Unger R, Isobe T, Yonath A, Michaeli S, A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei. Nat Commun, 14(1):7462(2023) [pubmed]
10.1038/s41467-023-43263-6;
Keyword List
submitter keyword: pseudouridine, RNA modification, snoRNA, quantitative proteomics,Trypanosome
Contact List
Prof. Shulamit Michaeli
contact affiliationBar-Ilan University
contact emailShulamit.Michaeli@biu.ac.il
lab head
Tirza Doniger
contact affiliationBar-Ilan University
contact emailtirza.doniger@biu.ac.il
dataset submitter
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Dataset FTP location
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