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PXD030540

PXD030540 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSRSF1 as a novel interacting partner for IFITM1/3 unravels the emergent role of IFITM1/3 mediating protein expression
DescriptionIFITM proteins play a role in cancer cell progression through undefined mechanisms. Recent data demonstrated that interferon induced transmembrane 1 and 3 (IFITM1/3) proteins are required for the interferon gamma (IFNγ) stimulated synthesis of a subset of IFN-stimulated proteins; including human leukocyte antigen B (HLA-B). SBP-tagged IFITM1 protein was used to identify an association of IFITM1 protein with the cytosolic isoform of serine and arginine rich splicing factor 1 (SRSF1). This cytosolic association was confirmed in situ using proximity ligation assays for SRSF1 and IFITM1/3 suggesting a role associated with translation. Accordingly, IFITM1/3 were shown to interact with HLA-B mRNA in response to IFNγ stimulation using RNA-protein proximity ligation assays. In addition, shotgun RNA sequencing in IFITM1/IFITM3 null cells and wt-SiHa cells indicated that reduced HLA-B gene expression does not account for lowered HLA-B protein synthesis in response to IFNγ. Furthermore, ribosome profiling by using sucrose gradient sedimentation identified a reduction in 80S ribosomal fraction an IFITM1/IFITM3 null cells compared to their wild-type counterpart, partially reverted by IFITM1/3 complementation. Taking all together, these data link the binding of IFITM1/3 proteins to HLA-B mRNA and SRSF1 as a mechanism to catalyze the synthesis of target proteins suggesting an RNA chaperonin role for IFITM1/3 proteins.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:16:32.173.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLenka Hernychova
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C labeled residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-20 02:11:25ID requested
12022-10-13 09:04:17announced
22023-11-14 08:16:33announced2023-11-14: Updated project metadata.
Publication List
G, ó, mez-Herranz M, Faktor J, Y, é, benes Mayordomo M, Pilch M, Nekulova M, Hernychova L, Ball KL, Vojtesek B, Hupp TR, Kote S, Emergent Role of IFITM1/3 towards Splicing Factor (SRSF1) and Antigen-Presenting Molecule (HLA-B) in Cervical Cancer. Biomolecules, 12(8):(2022) [pubmed]
Keyword List
submitter keyword: mass spectrometry, interferon, ribosome, isoform of serine and arginine rich splicing factor 1,CRISPR/Cas, mRNA, interferon induced transmembrane 1 and 3
Contact List
Prof. Ted Hupp
contact affiliationThe University of Edinburgh
contact emailTed.Hupp@ed.ac.uk
lab head
Lenka Hernychova
contact affiliationMasaryk Memorial Cancer Institutre, Zluty kopec 7, Brno 656 53, Czech Republic
contact emaillenka.hernychova@mou.cz
dataset submitter
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Dataset FTP location
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