PXD030534

PXD030534 is an original dataset announced via ProteomeXchange.

Title	Proteomic investigation into the action mechanism of inhibitor ptsc1 against Streptococcus pneumoniae
Description	Phenyl-thiazolylurea-sulfonamides compound 1 (ptsc1) is a new and efficient bacterial inhibitor. Although the inhibitor has antibacterial activity against several model bacteria, the details of its mechanism in Streptococcus pneumoniae (S. pneumoniae) have not been revealed. In the current study, we found that ptsc1 can significantly inhibit the growth of S. pneumoniae. To further investigate the molecular mechanism of its antibacterial effects, quantitative proteomics was performed to identify differential expressed proteins in S. pneumoniae. Compared with the normal group, the expression levels of 38 proteins were upregulated, whereas those of 88 proteins were downregulated (> 1.3 fold-change) in the experimental group (P < 0.05). KEGG pathways analysis revealed that ptsc1 regulated proteins were mainly involved in carbohydrate metabolism, DNA replication and repair, pyrimidine metabolism, fatty acid biosynthesis and oxidative phosphorylation pathways. The results of GO enrichment analysis showed that the inhibitor could lead to dysregulation of bacterial translation and energy metabolism, and cause intracellular oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2022-01-12
AnnouncementXML	Submission_2022-01-11_19:08:10.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kun Cao
SpeciesList	scientific name: Streptococcus pneumoniae D39; NCBI TaxID: 373153;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-20 01:53:59	ID requested
⏵ 1	2022-01-11 19:08:10	announced

Dataset with its publication pending

submitter keyword: S. pneumoniae, tRNA binding, ROS, Bacteriostasis

Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics
contact affiliation	Guangdong Medical University, Dongguan, China.
contact email	caokun@gdmu.edu.cn
lab head
Kun Cao
contact affiliation	Guangdong medical University,dongguan, GUANGDONG
contact email	caokun@gdmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD030534

PRIDE project URI

• PRIDE
  1. PXD030534
     1. Label: PRIDE project
     2. Name: Proteomic investigation into the action mechanism of inhibitor ptsc1 against Streptococcus pneumoniae