PXD030527

PXD030527 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MITF deficiency accelerates GNAQ-driven uveal melanoma
Description	Cutaneous melanoma (CM) and uveal melanoma (UM) both originate from the melanocytic lineage but are primarily driven by distinct oncogenic drivers, BRAF/NRAS or GNAQ/GNA11 respectively. The melanocytic master transcriptional regulator, MITF, is essential for both CM development and maintenance, but its role in UM is largely unexplored. Here, we use zebrafish models to dissect the key UM oncogenic signaling events, and establish the role of MITF in UM tumors. Remarkably, mitfa deficiency was profoundly UM promoting, dramatically accelerating the onset and progression of tumors induced by Tg(mitfa:GNAQQ209L);tp53M214K/M214K. To further explore the role of MITF in GNAQ-driven tumorigenesis, we performed phospho-proteomics and total proteomics on 5 zebrafish GNAQ Tg(mitfa:GNAQQ209L);tp53M214K/M214K tumors and 5 zebrafish GNAQ Tg(mitfa:GNAQQ209L);tp53M214K/M214K;mitfa-/- tumors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:45.700.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030527
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Grace Phelps
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue
Instrument	Orbitrap Exploris 240

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-19 04:46:24	ID requested
1	2023-03-11 01:07:37	announced
⏵ 2	2023-11-14 08:39:46	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD030527;
Phelps GB, Hagen HR, Amsterdam A, Lees JA, MITF deficiency accelerates GNAQ-driven uveal melanoma. Proc Natl Acad Sci U S A, 119(19):e2107006119(2022) [pubmed]

10.6019/PXD030527;

Phelps GB, Hagen HR, Amsterdam A, Lees JA, MITF deficiency accelerates GNAQ-driven uveal melanoma. Proc Natl Acad Sci U S A, 119(19):e2107006119(2022) [pubmed]

Keyword List

submitter keyword: uveal melanoma, phospho, mitf,Zebrafish

submitter keyword: uveal melanoma, phospho, mitf,Zebrafish

Contact List

Jacqueline A. Lees
contact affiliation	Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
contact email	jalees@mit.edu
lab head
Grace Phelps
contact affiliation	Massachusetts Institute of Technology
contact email	gbphelps@mit.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030527
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030527

PRIDE project URI

Repository Record List

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