PXD030522

PXD030522 is an original dataset announced via ProteomeXchange.

Title	Whole eye proteomic analysis of bbs1 zebrafish mutants
Description	Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal dystrophy, intellectual disability, polydactyly, obesity and renal anomalies. In photoreceptors (PR), light sensation occurs in outer segments (OSs), which are specialized primary cilia. BBS1, the major BBS gene, is part of a protein complex called “BBSome”, which is involved in intracellular protein transport. However, the precise function of BBS1/BBSome in controlling trafficking of ciliary proteins in PRs remains unclear. We generated a bbs1 knock out zebrafish mutant to investigate the role of Bbs1 and the BBSome in vivo. To detect global changes in the eye proteome and to verify the knock-out of Bbs1, we conducted a whole eyes proteomic investigation.
HostingRepository	PRIDE
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-02-10_14:21:11.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Masek
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	methionine oxidation with neutral loss of 80 Da; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-19 04:31:58	ID requested
⏵ 1	2022-02-10 14:21:12	announced

Dataset with its publication pending

submitter keyword: Zebrafish, Eye, BBS1, LC-MS/MS

Ruxandra Bachmann-Gagescu
contact affiliation	Institute of Medical Genetics, Bachmann-Lab, University of Zurich, Switzerland
contact email	ruxandra.bachmann@mls.uzh.ch
lab head
Markus Masek
contact affiliation	University of Zurich
contact email	markus.masek@uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030522

PRIDE project URI

• PRIDE
  1. PXD030522
     1. Label: PRIDE project
     2. Name: Whole eye proteomic analysis of bbs1 zebrafish mutants