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PXD030511

PXD030511 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA cell-sorting based nano-scale pipeline for cell type-specific multi-omics in plants
DescriptionMulticellular organisms such as plants contain different types of cells with specialized functions. Analyzing the characteristics of each type of cells will reveal specific cell functions and enhance understanding of how an organism organizes and works. Here we report a high-sensitive and efficient cell-type-specific multi-omics pipeline, combining simplified flow cytometry-based fluorescent cell-sorting for fluorescent protoplasts and optimized nano-scale proteomics and metabolomics methods, which allow us to in-depth analyze the proteomics and metabolomics of a particular type of cells. By this method, we quantitatively compared the proteomics and metabolomics between guard cells and mesophyll cells and revealed that the enrichment of signal transduction-related proteins enables guard cells to respond to various environmental stimuli quickly. We uncovered a guard-cell-specific kinase cascade consisting of RAF15 and OST1 mediates the ABA-induced stomatal closure in guard cells. This pipeline is applicable to various types of cells in plant or non-plant systems to acquire systemic knowledge of how cells work specifically and in highly organized multiple cell organisms.
HostingRepositoryjPOST
AnnounceDate2022-12-18
AnnouncementXMLSubmission_2022-12-17_07:00:05.490.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHongliang Wang
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxymethyl-L-cysteine; alpha-amino acetylated residue
Instrumentinstrument; instrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-18 02:06:11ID requested
12022-12-17 07:00:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, metabolomics, single-cell, guard cell, abscisic acid, phosphorylation
Contact List
Pengcheng Wang
lab head
Hongliang Wang
contact affiliationShanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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