PXD030511
PXD030511 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A cell-sorting based nano-scale pipeline for cell type-specific multi-omics in plants |
Description | Multicellular organisms such as plants contain different types of cells with specialized functions. Analyzing the characteristics of each type of cells will reveal specific cell functions and enhance understanding of how an organism organizes and works. Here we report a high-sensitive and efficient cell-type-specific multi-omics pipeline, combining simplified flow cytometry-based fluorescent cell-sorting for fluorescent protoplasts and optimized nano-scale proteomics and metabolomics methods, which allow us to in-depth analyze the proteomics and metabolomics of a particular type of cells. By this method, we quantitatively compared the proteomics and metabolomics between guard cells and mesophyll cells and revealed that the enrichment of signal transduction-related proteins enables guard cells to respond to various environmental stimuli quickly. We uncovered a guard-cell-specific kinase cascade consisting of RAF15 and OST1 mediates the ABA-induced stomatal closure in guard cells. This pipeline is applicable to various types of cells in plant or non-plant systems to acquire systemic knowledge of how cells work specifically and in highly organized multiple cell organisms. |
HostingRepository | jPOST |
AnnounceDate | 2022-12-18 |
AnnouncementXML | Submission_2022-12-17_07:00:05.490.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hongliang Wang |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: cellular organisms; NCBI TaxID: 131567; |
ModificationList | S-carboxymethyl-L-cysteine; alpha-amino acetylated residue |
Instrument | instrument; instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-12-18 02:06:11 | ID requested | |
⏵ 1 | 2022-12-17 07:00:05 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, metabolomics, single-cell, guard cell, abscisic acid, phosphorylation |
Contact List
Pengcheng Wang | |
---|---|
lab head | |
Hongliang Wang | |
contact affiliation | Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001423/ |