PXD030511

PXD030511 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A cell-sorting based nano-scale pipeline for cell type-specific multi-omics in plants
Description	Multicellular organisms such as plants contain different types of cells with specialized functions. Analyzing the characteristics of each type of cells will reveal specific cell functions and enhance understanding of how an organism organizes and works. Here we report a high-sensitive and efficient cell-type-specific multi-omics pipeline, combining simplified flow cytometry-based fluorescent cell-sorting for fluorescent protoplasts and optimized nano-scale proteomics and metabolomics methods, which allow us to in-depth analyze the proteomics and metabolomics of a particular type of cells. By this method, we quantitatively compared the proteomics and metabolomics between guard cells and mesophyll cells and revealed that the enrichment of signal transduction-related proteins enables guard cells to respond to various environmental stimuli quickly. We uncovered a guard-cell-specific kinase cascade consisting of RAF15 and OST1 mediates the ABA-induced stomatal closure in guard cells. This pipeline is applicable to various types of cells in plant or non-plant systems to acquire systemic knowledge of how cells work specifically and in highly organized multiple cell organisms.
HostingRepository	jPOST
AnnounceDate	2022-12-18
AnnouncementXML	Submission_2022-12-17_07:00:05.490.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongliang Wang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxymethyl-L-cysteine; alpha-amino acetylated residue
Instrument	instrument; instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-18 02:06:11	ID requested
⏵ 1	2022-12-17 07:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: proteomics, metabolomics, single-cell, guard cell, abscisic acid, phosphorylation

submitter keyword: proteomics, metabolomics, single-cell, guard cell, abscisic acid, phosphorylation

Contact List

Pengcheng Wang
lab head
Hongliang Wang
contact affiliation	Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001423/