PXD030495

PXD030495 is an original dataset announced via ProteomeXchange.

Title	Identification of synaptic DGKq interactors that stimulate DGKq activity
Description	Lipids and their metabolic enzymes are a critical point of regulation for the membrane curvature required to induce membrane fusion during synaptic vesicle recycling. One such enzyme is diacylglycerol kinase theta (DGKq), which produces phosphatidic acid (PtdOH) that generates negative membrane curvature. Synapses lacking DGKq have significantly slower rates of endocytosis, implicating DGKq as an endocytic regulator. Importantly, DGKq kinase activity is required for this function. However, protein regulators of DGKq’s kinase activity in neurons have never been identified. In this study, we employed APEX2 proximity labeling and mass spectrometry to identify endogenous interactors of DGKq in neurons and assayed their ability to modulate its kinase activity. Seven endogenous DGKq interactors were identified and notably, synaptotagmin-1 (Syt1) increased DGKq kinase activity 10-fold. This study is the first to validate endogenous DGKq interactors at the mammalian synapse and suggests a coordinated role between DGKq-produced PtdOH and Syt1 in synaptic vesicle recycling.
HostingRepository	PRIDE
AnnounceDate	2021-12-18
AnnouncementXML	Submission_2021-12-17_16:32:40.672.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Casey Barber
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 13:22:27	ID requested
⏵ 1	2021-12-17 16:32:41	announced

Dataset with its publication pending

submitter keyword: DGK, Syt1, APEX2, LC-MSMS

Daniel M. Raben
contact affiliation	The Johns Hopkins University School of Medicine, Department of Biological Chemistry
contact email	draben@jhmi.edu
lab head
Casey Barber
contact affiliation	The Johns Hopkins University School of Medicine
contact email	cbarber9@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030495
     1. Label: PRIDE project
     2. Name: Identification of synaptic DGKq interactors that stimulate DGKq activity