PXD030484

PXD030484 is an original dataset announced via ProteomeXchange.

Title	Adrenaline-mediated activation of antibiotic production in Streptomyces highlights catechol as elicitor of specialized metabolism
Description	Actinobacteria are a rich source of bioactive molecules, and genome sequencing has shown that the vast majority of their biosynthetic potential has yet to be explored. However, many of their biosynthetic gene clusters (BGCs) are poorly expressed in the laboratory, which prevents discovery of their cognate natural products. To exploit their full biosynthetic potential, better understanding of the signals that promote the expression of BGCs is needed. Here, we show that the human stress hormone epinephrine (adrenaline) elicits antibiotic production by Actinobacteria. Catechol was established as the likely eliciting moiety, since similar responses were seen for catechol and for the catechol-containing molecules dopamine and catechin but not for related molecules. Exploration of the catechol-responsive strain Streptomyces sp. MBT84 using mass spectral networking revealed elicitation of a BGC that produces the angucycline glycosides aquayamycin, urdamycinone B and galtamycin C. Heterologous expression of the catechol-cleaving enzymes catechol 1,2-dioxygenase or catechol 2,3 dioxygenase counteracted the eliciting effect of catechol. Thus, for the first time we show the activation of natural product biosynthesis by a human hormone, leading to the identification of the ubiquitous catechol moiety as elicitor of BGCs for siderophores and antibiotics.
HostingRepository	PRIDE
AnnounceDate	2022-01-10
AnnouncementXML	Submission_2022-01-10_00:32:04.041.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030484
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chao DU
SpeciesList	scientific name: Streptomyces sp. MBT84; NCBI TaxID: 1488414;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 04:51:31	ID requested
⏵ 1	2022-01-10 00:32:04	announced

Dataset with its publication pending

submitter keyword: streptomyces, catechol, antibacteria, BGCs, MBT84

Prof.Dr. G.P. van Wezel
contact affiliation	Institute of Biology Leiden, Leiden University
contact email	g.wezel@biology.leidenuniv.nl
lab head
Chao DU
contact affiliation	Leiden University
contact email	c.du@biology.leidenuniv.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030484
     1. Label: PRIDE project
     2. Name: Adrenaline-mediated activation of antibiotic production in Streptomyces highlights catechol as elicitor of specialized metabolism