PXD030482

PXD030482 is an original dataset announced via ProteomeXchange.

Title	Plasma proteomics data of hibernating and active wild Scandinavian brown bears
Description	Here we provide mass-spectrometry based plasma proteomics data of hibernating and active wild Scandinavian brown bears (Ursus arctos arctos). The brown bear hibernates for half the year. Despite obesity and the prolonged period of inactivity, bears show no signs of the harmful effects associated with these conditions in humans. Thus, the hibernating bear is a protentional translational model for addressing these complications in humans. We analyzed plasma samples from 14 subadult 2- to 3-year-old (y/o) bears (6 males and 8 females) collected both during hibernation and active state, and for some for the bears during two seasons, resulting in a total of 38 analyzed plasma samples. In triplicates, the proteins in the plasma samples were unfolded and reduced. To increase depth of the analysis and the chance to detect low molecular weight proteins and peptides, we filtered samples with a 50K MWCO filter with the aim to deplete larger proteins. The proteins in the permeate were then tryptically digested, desalted, and analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed with the MaxQuant software searching against an Ursus arctos horribilis protein database.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:53.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Mette Frøbert
SpeciesList	scientific name: Ursus arctos arctos (European brown bear); NCBI TaxID: 563924;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 04:45:57	ID requested
1	2021-12-21 02:55:39	announced
2	2022-02-24 08:56:04	announced	2022-02-24: Updated publication reference for DOI(s): doi.org/10.1016/j.dib.2022.107959.
⏵ 3	2023-11-14 08:53:54	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ursus arctos,Brown Bear, Plasma, Proteomics, Hibernation, LC-MS/MS

Michael Toft Overgaard
contact affiliation	Department of Chemistry and Bioscience, Faculty of Engineering and Science, Aalborg University, Denmark
contact email	mto@bio.aau.dk
lab head
Anne Mette Frøbert
contact affiliation	Department of Chemistry and Bioscience, Aalborg University
contact email	amf@bio.aau.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD030482

PRIDE project URI

• PRIDE
  1. PXD030482
     1. Label: PRIDE project
     2. Name: Plasma proteomics data of hibernating and active wild Scandinavian brown bears