PXD030457

PXD030457 is an original dataset announced via ProteomeXchange.

Title	Proteomics of isolated sieve tubes from N.tabacum
Description	Symplasmicly connected cells called sieve elements form a network of tubes in the phloem of vascular plants. Sieve elements have essential functions as they provide routes for photoassimilate distribution, the exchange of developmental signals, and the coordination of defense responses. Nonetheless, they are the least understood main type of plant cells. They are extremely sensitive, possess a reduced endomembrane system without Golgi apparatus, and lack nuclei and translation machineries, so that transcriptomics and similar techniques cannot be applied. Moreover, the analysis of phloem exudates as a proxy for sieve element composition is marred by methodological problems. We developed a simple protocol for the isolation of sieve elements from leaves and stems of Nicotiana tabacum at sufficient amounts for large-scale proteome analysis. By quantifying the enrichment of individual proteins in purified sieve element relative to bulk phloem preparations, proteins of increased likelyhood to function specifically in sieve elements were identified. To evaluate the validity of this approach, YFP-constructs of genes encoding three of the candidate proteins were expressed in plants. Tagged proteins occurred exclusively in sieve elements. Two of them, a putative cytochrome b561/ferric reductase and a reticulon-like protein, appeared restricted to ER segments that were inaccessible to the luminal ER marker, HDEL-GFP, suggesting a previously unknown differentiation of the endomembrane system in sieve elements. Evidently, our list of promising candidate proteins provides a valuable exploratory tool for sieve element biology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:53.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joerg Hofmann
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 12:10:44	ID requested
1	2022-02-17 09:10:21	announced
⏵ 2	2024-10-22 05:32:55	announced	2024-10-22: Updated project metadata.

10.1073/pnas.2112755119;

Liu Y, Vasina VV, Kraner ME, Peters WS, Sonnewald U, Knoblauch M, : sieve element-specific proteins reveal differentiation of the endomembrane system. Proc Natl Acad Sci U S A, 119(1):(2022) [pubmed]

submitter keyword: proteomics,n.tabacum, sieve tubes

Michael Knoblauch
contact affiliation	School of Biological Sciences, Washington State University, Pullman, WA 99154, USA
contact email	knoblauch@wsu.edu
lab head
Joerg Hofmann
contact affiliation	FAU Erlangen-Nürnberg
contact email	joerg.hofmann@fau.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030457

PRIDE project URI

• PRIDE
  1. PXD030457
     1. Label: PRIDE project
     2. Name: Proteomics of isolated sieve tubes from N.tabacum