PXD030457 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics of isolated sieve tubes from N.tabacum |
Description | Symplasmicly connected cells called sieve elements form a network of tubes in the phloem of vascular plants. Sieve elements have essential functions as they provide routes for photoassimilate distribution, the exchange of developmental signals, and the coordination of defense responses. Nonetheless, they are the least understood main type of plant cells. They are extremely sensitive, possess a reduced endomembrane system without Golgi apparatus, and lack nuclei and translation machineries, so that transcriptomics and similar techniques cannot be applied. Moreover, the analysis of phloem exudates as a proxy for sieve element composition is marred by methodological problems. We developed a simple protocol for the isolation of sieve elements from leaves and stems of Nicotiana tabacum at sufficient amounts for large-scale proteome analysis. By quantifying the enrichment of individual proteins in purified sieve element relative to bulk phloem preparations, proteins of increased likelyhood to function specifically in sieve elements were identified. To evaluate the validity of this approach, YFP-constructs of genes encoding three of the candidate proteins were expressed in plants. Tagged proteins occurred exclusively in sieve elements. Two of them, a putative cytochrome b561/ferric reductase and a reticulon-like protein, appeared restricted to ER segments that were inaccessible to the luminal ER marker, HDEL-GFP, suggesting a previously unknown differentiation of the endomembrane system in sieve elements. Evidently, our list of promising candidate proteins provides a valuable exploratory tool for sieve element biology. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:32:53.603.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joerg Hofmann |
SpeciesList | scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-16 12:10:44 | ID requested | |
1 | 2022-02-17 09:10:21 | announced | |
⏵ 2 | 2024-10-22 05:32:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1073/pnas.2112755119; |
Liu Y, Vasina VV, Kraner ME, Peters WS, Sonnewald U, Knoblauch M, : sieve element-specific proteins reveal differentiation of the endomembrane system. Proc Natl Acad Sci U S A, 119(1):(2022) [pubmed] |
Keyword List
submitter keyword: proteomics,n.tabacum, sieve tubes |
Contact List
Michael Knoblauch |
contact affiliation | School of Biological Sciences, Washington State University, Pullman, WA 99154, USA |
contact email | knoblauch@wsu.edu |
lab head | |
Joerg Hofmann |
contact affiliation | FAU Erlangen-Nürnberg |
contact email | joerg.hofmann@fau.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030457
- Label: PRIDE project
- Name: Proteomics of isolated sieve tubes from N.tabacum