PXD030451

PXD030451 is an original dataset announced via ProteomeXchange.

Title	An mTORC1-CK2 axis controls starvation induced ER-phagy via FAM134C
Description	The degradation of endoplasmic reticulum (ER) via selective autophagy is driven by the ER-phagy receptors that facilitate the incorporation of ER-fragments into nascent autophagosomes. How these receptors are regulated, in response to ER-phagy-inducing stimuluses, is largely unknown. Here we propose that starvation, as well as mTOR inhibition, triggers ER-phagy primarily through the activation of the ER-phagy receptor FAM134C. In physiological, nutrient reach, conditions FAM134C is phosphorylated by the Casein kinase 2 (CK2) protein at specific residues negatively affecting FAM134C interaction with the LC3 proteins, thereby preventing ER-phagy. Pharmacological or starvation-induced mTORC1 inhibition limits phosphorylation of FAM134C by CK2, hence promoting FAM134C activation and ER-phagy. Moreover, inhibition of CK2 or the expression of a phospho-mutant FAM134C protein is sufficient to stimulate ER-phagy. Conversely, starvation induced ER-phagy is inhibited in cells and mice that lack FAM134C or expressing a phospho-mimetic FAM134C protein. Overall, these data describe a new mechanism regulating ER-phagy and provides an example of cargo selectivity mechanism during starvation induced autophagy.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:18:32.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giorgia Di Lorenzo
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 11:52:36	ID requested
1	2022-10-14 02:43:52	announced
⏵ 2	2023-11-14 07:18:35	announced	2023-11-14: Updated project metadata.

Di Lorenzo G, Iavarone F, Maddaluno M, Plata-G, ó, mez AB, Aureli S, Quezada Meza CP, Cinque L, Palma A, Reggio A, Cirillo C, Sacco F, Stolz A, Napolitano G, Marin O, Pinna LA, Ruzzene M, Limongelli V, Efeyan A, Grumati P, Settembre C, Phosphorylation of FAM134C by CK2 controls starvation-induced ER-phagy. Sci Adv, 8(35):eabo1215(2022) [pubmed]

submitter keyword: Human,FAM134C,autophagy,rat,CK2,mTORC1,ER-phagy

Carmine Settembre
contact affiliation	Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy Department of Clinical Medicine and Surgery, Federico II University, Naples, Italy
contact email	settembre@tigem.it
lab head
Giorgia Di Lorenzo
contact affiliation	Telethon Institute of Genetics and Medicine (TIGEM)
contact email	g.dilorenzo@tigem.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD030451

PRIDE project URI

• PRIDE
  1. PXD030451
     1. Label: PRIDE project
     2. Name: An mTORC1-CK2 axis controls starvation induced ER-phagy via FAM134C