PXD030448

PXD030448 is an original dataset announced via ProteomeXchange.

Title	A systematic screening pipeline identifies a new regulator of tyrosinated microtubule dynamics
Description	Tubulin detyrosination is a reversible post-translational modification thought to be important for processes including the generation of cell polarity and cell division. The Y/ΔY cycle does not affect the intrinsic properties of microtubules per se, but rather influences the cohort of microtubule-associated proteins (MAPs) and motor proteins that associate with microtubules. Here we present a screening pipeline to identify proteins that bind microtubules in a manner that depends on the Y versus ΔY state.
HostingRepository	PRIDE
AnnounceDate	2022-08-16
AnnouncementXML	Submission_2022-08-15_19:56:00.844.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Haynes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 11:17:19	ID requested
⏵ 1	2022-08-15 19:56:02	announced

Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R, EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Curr Biol, 32(18):3898-3910.e14(2022) [pubmed]

submitter keyword: microtubule, tyrosination, detyrosination, TMT6, LC-MS/MS, human

Alexey Nesvizhskii
contact affiliation	Department of Pathology, University of Michigan, USA
contact email	nesvi@med.umich.edu
lab head
Sarah Haynes
contact affiliation	University of Michigan
contact email	hayse@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030448

PRIDE project URI

• PRIDE
  1. PXD030448
     1. Label: PRIDE project
     2. Name: A systematic screening pipeline identifies a new regulator of tyrosinated microtubule dynamics