PXD030441

PXD030441 is an original dataset announced via ProteomeXchange.

Title	Cycloastragenol inhibit experimental abdominal aortic aneurysm progression
Description	Abstract: The pathogenesis of AAA involves vascular inflammation and oxidative stress. Astragali Radix contains cycloastragenol (CAG) known to have anti-inflammatory and anti-oxidative properties. We hypothesized that CAG supplement impairs AAA progression. AAA was induced in male rats by intraluminal elastase infusion in the infrarenal aorta and treated daily with CAG (125 mg/kg/day). Aortic expansion was followed weekly by ultrasound, with euthanization at day 28. Changes in AAA wall composition were analyzed at mRNA levels, histology, zymography and explorative proteomic analyses. At day 28, mean AAA diameter was 37% lower in CAG group (p<0.0001). In aneurysm cross sections, elastin content was insignificantly higher in CAG group (10.5% ± 5.9% vs 19.9% ± 16.8%, p=0.20) with more preserved elastin lamellae structures (p=0.0003) and with no microcalcifications. Aneurysmal matrix metalloprotease-2 activity was reduced by CAG treatment (p=0.022), and mRNA levels of inflammatory- and antioxidative markers showed no difference between groups. Explorative proteomic analysis showed no difference in protein levels when adjustment for multiple testing. Amongst unadjusted affected proteins were fibulin-5 (p=0.02), aquaporin-1 (p=0.02) and prostacyclin synthase (p=0.006). CAG impairs experimental AAA progression by reduction of elastin degradation through decreased MMP-2 activity, thus CAG could be considered tested in AAA patients.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_04:09:10.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lasse Steffensen
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-16 02:56:22	ID requested
⏵ 1	2022-05-20 04:09:11	announced

Melin LG, Dall JH, Lindholt JS, Steffensen LB, Beck HC, Elkrog SL, Clausen PD, Rasmussen LM, Stubbe J, Cycloastragenol Inhibits Experimental Abdominal Aortic Aneurysm Progression. Biomedicines, 10(2):(2022) [pubmed]

submitter keyword: aortic aneurysm

pathogenesis

pharmacological therapy

experimental model

drug delivery

Hans C Beck
contact affiliation	Odense University Hospital/University of Southern Denmark
contact email	hans.christian.beck@rsyd.dk
lab head
Lasse Steffensen
contact affiliation	University of Southren Denmark
contact email	lsteffensen@health.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030441
     1. Label: PRIDE project
     2. Name: Cycloastragenol inhibit experimental abdominal aortic aneurysm progression