PXD030403 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Thyroid Hormone Dependent Transcriptional Programming by TRb Requires SWI/SNF Chromatin Remodelers |
Description | Transcriptional regulation in response to thyroid hormone (3,5,3ยด-triiodo-L-thyronine, T3) is a dynamic and cell-type specific process that maintains cellular homeostasis and identity in all tissues. However, our understanding of the mechanisms of thyroid hormone receptor (TR) actions at the molecular level are actively being refined. We used an integrated genomics approach to profile and characterize the cistrome of TRb, map changes in chromatin accessibility, and capture the transcriptomic changes in response to T3 in normal human thyroid cells. There are significant shifts in TRb genomic occupancy in response to T3, which are associated with differential chromatin accessibility, and differential recruitment of SWI/SNF chromatin remodelers. We further demonstrate selective recruitment of BAF and PBAF SWI/SNF complexes to TRb binding sites, revealing novel differential functions in regulating chromatin accessibility and gene expression. Our findings highlight three distinct modes of TRb interaction with chromatin and coordination of coregulator activity. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-25 |
AnnouncementXML | Submission_2022-05-25_04:56:06.509.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Noelle Gillis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-14 09:55:13 | ID requested | |
⏵ 1 | 2022-05-25 04:56:07 | announced | |
Publication List
Gillis NE, Boyd JR, Tomczak JA, Frietze S, Carr FE, requires SWI/SNF chromatin remodelers. Nucleic Acids Res, 50(3):1382-1395(2022) [pubmed] |
Keyword List
submitter keyword: thyroid hormone receptor, transcription, coregulators, nuclear receptor |
Contact List
Frances Carr |
contact affiliation | Department of Pharmacology, University of Vermont Larner College of Medicine |
contact email | fcarr@uvm.edu |
lab head | |
Noelle Gillis |
contact affiliation | University of Vermont |
contact email | negillis@uvm.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030403
- Label: PRIDE project
- Name: Thyroid Hormone Dependent Transcriptional Programming by TRb Requires SWI/SNF Chromatin Remodelers