PXD030401 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discrimination of Tilletia controversa from the T. caries / T. laevis complex by MALDI-TOF MS analysis of teliospores |
Description | It is of high importance to distinguish Tilletia caries and Tilletia laevis as causal agents of common bunt accurately from Tilletia controversa, the causal agent of dwarf bunt. All three of these wheat bunt diseases can lead to significant yield losses in crop production worldwide. But T. controversa is categorized as a quarantine pest in most areas of the world and must be discriminated from the T. caries / T. laevis complex. Usually, morphological characteristics of the teliospores are used to differentiate the three species. But due to natural hybridization and overlapping properties the discrimination is challenging. Germination behavior can also be considered for discrimination, but equivalent to their similar physiological and genetic traits the two agents of common bunt, T. caries and T. laevis could not be distinguished by this. It was suggested that the two species and maybe all three of those described Tilletia species might be conspecific. Up to now no molecular based method is available to differentiate the three species. Several studies have attempted the detection of the wheat bunt Tilletia species using PCR or other DNA-based methods. Other studies analyzed protein patterns with electrophoresis methods. But none of these approaches was able to distinguish between all of the three closely related Tilletia species. Several studies have shown that Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) is a useful tool to differentiate closely related fungal species. The aim of this study was to assess whether MALDI-TOF MS analysis is able to distinguish specimens of the three closely related pathogens T. caries, T. laevis, and T. controversa and may constitute an alternative method to the usually used morphology-based identification. Therefore MALDI-TOF MS was used to create subproteome fingerprints of the teliospores of 69 Tilletia specimens. These fingerprints were analyzed by comparing the mass spectra to each other by high-throughput multidimensional scaling (HiT-MDS ) together with hierarchical cluster analysis (HCA). The second approach was performed by discriminant analysis of principal components (DAPC). MALDI-TOF MS has proven to be a useful method for distinguishing between T. controversa and the two causal agents of common bunt, using our developed method of direct analysis of teliospores, but was unable to separate T. caries and T. laevis species. We conclude a potentially conspecific status of T. caries and T. laevis or even two morphotypes of one common species, causing identical disease symptoms and sharing the same germination requirements along with a related protein composition, shown in this study. Our developed MALDI-TOF MS method can be helpful in testing Tilletia bunt balls collected during field inspections, especially with regard to quarantine regulations or for breeding applications and may also be transferred to analyze further challenging sample material. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:31:11.940.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Monika Forster |
SpeciesList | scientific name: Tilletia caries; NCBI TaxID: 13290; scientific name: Tilletia laevis; NCBI TaxID: 157183; scientific name: Tilletia indica; NCBI TaxID: 43049; scientific name: Tilletia controversa; NCBI TaxID: 13291; |
ModificationList | No PTMs are included in the dataset |
Instrument | microflex LT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-14 09:52:41 | ID requested | |
1 | 2022-02-14 01:27:53 | announced | |
⏵ 2 | 2024-10-22 05:31:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1007/s00253-021-11757-2; |
Forster MK, Sedaghatjoo S, Maier W, Killermann B, Niessen L, Discrimination of Tilletia controversa from the T. caries/T. laevis complex by MALDI-TOF MS analysis of teliospores. Appl Microbiol Biotechnol, 106(3):1257-1278(2022) [pubmed] |
Keyword List
submitter keyword: MALDI-TOF MS, Tilletia caries,Tilletia controversa, Tilletia laevis |
Contact List
Niessen Ludwig Martin |
contact affiliation | Technical University of Munich TUM School of Life Sciences Chair of Microbiologie Germany |
contact email | ludwig.niessen@tum.de |
lab head | |
Monika Forster |
contact affiliation | Bavarian State Research Center for Agriculture |
contact email | monikaforster@online.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030401 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030401
- Label: PRIDE project
- Name: Discrimination of Tilletia controversa from the T. caries / T. laevis complex by MALDI-TOF MS analysis of teliospores